Method for Reducing Sequencing Errors Caused by DNA Fragmentation
AssigneeNew England Biolabs, Inc.
InventorsThomas C. Evans, Jr., Laurence Ettwiller, Lixin Chen, Pingfang Liu
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Abstract
Provided herein, among other things, is a method for reducing sequencing errors caused by mechanical DNA fragmentation. In some embodiments, this method may comprise: (a) obtaining a DNA sample; (b) mechanically fragmenting the DNA sample to produce a template; (c) treating the fragmented DNA with a plurality of DNA repair enzymes; and (d) obtaining a sequence of the fragmented DNA. The treatment step (c) reduces the number of fragmentation induced errors.
Claims16
A method for reducing sequencing errors caused by mechanical DNA fragmentation, comprising:
- (a) obtaining a DNA sample;
- (b) mechanically fragmenting the DNA sample to produce a template;
- (c) treating the fragmented DNA with a plurality of DNA repair enzymes;
- and
- (d) obtaining a sequence of the fragmented DNA,
- wherein step (c) reduces the number of fragmentation induced errors.
A method for detecting damaged DNA in a sample, comprising:
- preparing a sequencing library from the damaged DNA;
- sequencing the sequencing library to obtain a plurality of first Watson end sequence reads (R1s) and plurality of second complementary Crick sequence reads (R2s);
- for a selected locus, identifying those R1s and R2s that have a sequence abnormality;
- and
- detecting an imbalance between the number of R1s that have a sequence abnormality and the number of R2s that have a complementary sequence abnormality at the same position.
Dependencies are inferred from the claim text.