Sharply resolving labeled protein molecular weight standards
AssigneeLife Technologies Corporation
InventorsRoumen BOGOEV, Douglas Kang
Life of this patent
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Abstract
Pre-labeled protein standards useful in electrophoresis that have sharp, consistent separation characteristics that are substantially the same as those of their unlabeled counterparts are provided. The invention provides pre-labeled protein standard sets that include a plurality of labeled proteins that are labeled on a first amino acid, in which side reactions of the label with amino acids not targeted for labeling are reduced.
Claims23
-94. (canceled)
A pre-labeled protein standard set comprising a plurality of labeled proteins, wherein one or more of the plurality of labeled proteins is a selectively labeled protein comprising a labeling compound on a first or a target amino acid and is either depleted in residues of a second or a non-target amino acid that can react with the labeling compound or includes an amino acid sequence having homology to a naturally-occurring protein which has fewer residues of the second or the non-target amino acid.
The pre-labeled protein standard set of claim 1, wherein the selectively labeled protein lacks residues of the second amino acid.
The pre-labeled protein standard set of claim 1, wherein the first or target amino acid is lysine and the second amino acid is one or more of cysteine, histidine, or tryptophan.
The pre-labeled protein standard set of claim 1, wherein the first amino acid is cysteine and the second amino acid is one or more of lysine, histidine, or tryptophan.
The pre-labeled protein standard set of claim 1, wherein the first amino acid is glutamate and the second amino acid is apartate.
The pre-labeled protein standard set of claim 1, wherein the first amino acid is aspartate and the second amino acid is glutamate.
The pre-labeled protein standard set of claim 1, wherein the first amino acid is histidine and the second amino acid is one or more of cysteine, lysine, or tryptophan.
The pre-labeled protein standard set of claim 1, wherein the first amino acid is tryptophan and the second amino acid is one or more of cysteine, lysine, histidine, or asparagine.
The pre-labeled protein standard set of claim 1, wherein the first amino acid is asparagine and the second amino acid is one or more or cysteine, lysine, histidine, or tryptophan.
The pre-labeled protein standard set of claim 1, wherein the first amino acid is tyrosine and the second amino acid is one or more of cysteine, lysine, histidine, or tryptophan.
The pre-labeled protein standard set of claim 1, wherein the first amino acid is methionine and the second amino acid is one or more of cysteine, lysine, histidine, tyrosine, or tryptophan.
The pre-labeled protein standard set of claim 1 having a molecular weight range of 3.5 kDa to 260 KDa.
The pre-labeled protein standard set of claim 1, wherein fewer than one residue of the second amino acid is present per ten kilodalton (kDa) molecular weight of the selectively labeled protein.
The pre-labeled protein standard set of claim 1, wherein the naturally-occurring protein is an ADP-ribosylation factor, a Bacillus megaterium spore protein, a seed storage protein, the L domain of HIV or Ebola viruses, or a WNK protein.
The pre-labeled protein standard set of claim 1, wherein the labeling compound is a chromophore, a fluorophore or a dye.
The pre-labeled protein standard set of claim 1, wherein the plurality of labeled proteins are provided in one or more solutions and wherein the one or more solutions comprise at least one buffer, at least one detergent, at least one reducing agent, at least one alcohol, or at least one dye.
A kit comprising:
- a pre-labeled protein standard set of claim 1;
- and
- one or more of:
- one or more gel cassettes, a gel loading buffer, a gel loading dye, a reducing agent, a blotting membrane, a lysis buffer, a purification matrix, or an unlabeled protein standard set.
Dependencies are inferred from the claim text.