# US 2018/0024147 A1 · Reagent kit for detecting sex hormone and method for detecting sex hormone using same > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2018/0024147 A1 - Document ID: US20180024147A1 - Status: Published application - Kind: A1 - Application number: 15/542,432 - Filed: 2016-01-06 - Published: 2018-01-25 - Earliest claimed: 2015-01-09 - Assignees: Indutry-University Cooperation Foundation Hanyang University Erica Campus; Eone Reference Laboratory - Applicants: Indutry-University Cooperation Foundation Hanyang University Erica Campus; Eone Reference Laboratory - Inventors: Jaebum CHOO; Hyangah CHON; Rui WANG; Sangyeop LEE; Young Ho YOON; Sung Hyun HONG - Web page: https://patentgrep.com/patent/US20180024147A1 ## CPC - G01N 33/743: {Steroid hormones} - G01N 21/658: {enhancement Raman, e.g. surface plasmons} - G01N 33/54326: {Magnetic particles} - G01N 33/588: {with semiconductor nanocrystal label, e.g. quantum dots} - G01N 2800/048: Pituitary or hypothalamic - pituitary relationships, e.g. vasopressin or ADH related - G01N 2800/38: Pediatrics - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract The present disclosure provides a reagent kit for detecting a sex hormone, which contains a first reagent containing a metal nanoprobe in which a sex hormone and a Raman reporter are immobilized and a second reagent containing a magnetic particle in which an antibody for detecting the sex hormone is immobilized, and a method for detecting a sex hormone using the same. ## Claims ### Claim 1 A reagent kit for detecting a sex hormone, which comprises: a first reagent comprising a metal nanoprobe in which a sex hormone and a Raman reporter are immobilized; and a second reagent comprising a magnetic particle in which an antibody for detecting the sex hormone is immobilized. ### Claim 2 (depends on 1) The reagent kit for detecting a sex hormone according to claim 1, wherein the antibody for detecting the sex hormone comprises a primary antibody and a secondary antibody, the secondary antibody is immobilized on the magnetic particle and the primary antibody binds to the secondary antibody and reacts with the sex hormone. ### Claim 3 (depends on 1) The reagent kit for detecting a sex hormone according to claim 1, wherein the sex hormone is estrogen or testosterone. ### Claim 4 (depends on 3) The reagent kit for detecting a sex hormone according to claim 3, wherein the estrogen is one or more selected from a group consisting of estradiol, estrone and estriol. ### Claim 5 (depends on 1) The reagent kit for detecting a sex hormone according to claim 1, wherein the detectable concentration of the sex hormone is 0.1-1,000 pg/mL. ### Claim 6 (depends on 1) The reagent kit for detecting a sex hormone according to claim 1, wherein the limit of detection of the sex hormone is 0.1 pg/mL. ### Claim 7 (depends on 1) The reagent kit for detecting a sex hormone according to claim 1, wherein the detection time of the sex hormone is 2 hours or shorter. ### Claim 8 A SERS-based method for detecting a sex hormone comprising the steps of: preparing a sample solution comprising a sex hormone; preparing a metal nanoprobe in which the sex hormone is bound to a Raman reporter; preparing a magnetic particle in which a primary antibody and a secondary antibody for detecting the sex hormone are immobilized; adding the metal nanoprobe and the magnetic particle in which the primary antibody and the secondary antibody are immobilized to the sample solution at the same time; forming an immunocomplex with the magnetic particle through a competitive immunoreaction of each of the sex hormone in the sample solution and the sex hormone of the metal nanoprobe with the primary antibody immobilized in the magnetic particle; separating the magnetic particle in which the immunocomplex is formed using magnetism; irradiating a laser light to the separated magnetic particle; and detecting the sex hormone by measuring a surface-enhanced Raman scattering (SERS) signal after the irradiation of the laser light. ### Claim 9 (depends on 8) The SERS-based method for detecting a sex hormone according to claim 8, wherein, in the step of forming the immunocomplex, the intensity of the SERS signal is decreased as the concentration of the sex hormone in the sample solution is higher because the amount of the metal nanoprobe forming the immunocomplex with the magnetic particle is decreased; or the intensity of the SERS signal is increased as the concentration of the sex hormone in the sample solution is lower because the amount of the metal nanoprobe forming the immunocomplex with the magnetic particle is increased. ### Claim 10 (depends on 8) The SERS-based method for detecting a sex hormone according to claim 8, wherein the sex hormone is estrogen or testosterone. ### Claim 11 (depends on 10) The SERS-based method for detecting a sex hormone according to claim 10, wherein the estrogen is one or more selected from a group consisting of estradiol, estrone and estriol. ### Claim 12 (depends on 8) The SERS-based method for detecting a sex hormone according to claim 8, wherein the sample solution is selected from a group consisting of a tissue extract, a cell lysate, a whole blood, a blood plasma, a blood serum, a saliva, an ocular fluid, a cerebrospinal fluid, a sweat, a urine, a milk, an ascitic fluid, a synovial fluid, a peritoneal fluid and a dried blood spot. ### Claim 13 (depends on 8) The SERS-based method for detecting a sex hormone according to claim 8, wherein the secondary antibody is an anti-mouse antibody immobilized on the magnetic particle and the primary antibody is an anti-sex hormone antibody which binds to the secondary antibody and specifically immunoreacts with the sex hormone. ### Claim 14 (depends on 8) The SERS-based method for detecting a sex hormone according to claim 8, wherein the detectable concentration of the sex hormone is 0.1-1,000 pg/mL. ### Claim 15 (depends on 8) The SERS-based method for detecting a sex hormone according to claim 8, wherein the limit of detection of the sex hormone is 0.1 pg/mL. ### Claim 16 (depends on 8) The SERS-based method for detecting a sex hormone according to claim 8, wherein the detection time of the sex hormone is 2 hours or shorter. ### Claim 17 (depends on 8) A method for diagnosis of precocious puberty of a subject comprising the steps of: extracting a sample solution from a subject; detecting a sex hormone for the extracted sample solution by the method for detecting a sex hormone according to claim 8; and determining the concentration of the detected sex hormone. ### Claim 18 (depends on 17) The method according to claim 17, wherein the concentration of the detected sex hormone is 0.1-1000 pg/mL. ### Claim 19 (depends on 17) The method according to claim 17, which further comprises, after the step of determining the concentration of the detected sex hormone, a step of diagnosing as precocious puberty when the concentration of the detected sex hormone is higher than 10 pg/mL.