# US 2018/0024134 A1 · Immobilization of cells or virus particles on protein structures using a microfluidic chamber > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2018/0024134 A1 - Document ID: US20180024134A1 - Status: Published application - Kind: A1 - Application number: 15/550,454 - Filed: 2016-02-05 - Published: 2018-01-25 - Earliest claimed: 2015-02-13 - Applicants: Karlsruher Institut Fuer Technologie; Universitaetsklinikum Hamburg-Eppendorf (UKE) - Inventors: Michael HIRTZ; Harald FUCHS; Klaus PANTEL; Falko BRINKMANN - Web page: https://patentgrep.com/patent/US20180024134A1 ## CPC - G01N 33/5759: {involving compounds localised on the membrane of tumour or cancer cells} - B01L 3/50273: {characterised by the means or forces applied to move the fluids} - B01L 3/502761: {specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules} - G01N 33/56966: {Animal cells} - G01N 33/56983: {Viruses} - B01L 2300/0819: Microarrays; Biochips - B01L 2300/18: Means for temperature control - B01L 2400/0406: capillary forces - C12N 7/02: Recovery or purification - G01N 2333/705: Assays involving receptors, cell surface antigens or cell surface determinants - G01N 2469/10: Detection of antigens from microorganism in sample from host - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) ## Abstract The present invention relates to methods for the immobilization of cells or virus particles of interest expressing one or more predetermined surface marker(s) on defined spots on a solid support, comprising the steps of providing a biological fluid sample suspected of containing cells or virus particles of interest; labeling the cells or virus particles of interest with antibodies, antibody fragments or antibody mimetics directed against the one or more predetermined surface marker(s) and carrying a first binding agent; and contacting the labeled cells or virus particles of interest with a solid support (biochip), said solid support comprising an array of defined isolated spots of a solid support-bound second binding agent, wherein the first and second binding agents can bind to each other. The present invention further relates to devices for the isolation of cells or virus particles of interest expressing one or more predetermined surface marker(s). ## Claims ### Claim 1 A method for the immobilization of cells or virus particles of interest expressing one or more predetermined surface marker(s) on defined spots on a solid support, comprising the steps: (a) providing a biological fluid sample suspected of containing cells or virus particles of interest; (b) labeling the cells or virus particles of interest with antibodies, antibody fragments or antibody mimetics directed against the one or more predetermined surface marker(s) and carrying a first binding agent; and (c) contacting the labeled cells or virus particles of interest with a solid support, said solid support comprising an array of defined isolated spots of a solid support-bound second binding agent, wherein the first and second binding agents can bind to each other. ### Claim 2 (depends on 1) The method according to claim 1, further comprising, after step (c), the step of identifying solid support-bound cells or virus particles of interest microscopically. ### Claim 3 (depends on 1) The method according to claim 1, further comprising, after step (c), the step of isolating one or more individual immobilized cells or virus particles from the solid support. ### Claim 4 (depends on 1) The method according to claim 1, wherein the biological fluid sample is selected from the group consisting of blood, blood components, amniotic fluid, cerebrospinal fluid, lymphatic fluid, peritoneal fluid, saliva, sputum, urine, and partitions or fractions thereof. ### Claim 5 (depends on 1) The method according to claim 1, wherein the cells are circulating tumor cells (CTCs). ### Claim 6 (depends on 1) The method according to claim 1, wherein the surface marker is Epithelial Cell Adhesion Molecule (EpCAM). ### Claim 7 (depends on 1) The method according to claim 1, wherein the second binding agent is fluorescently labeled. ### Claim 8 (depends on 1) The method according to claim 1, wherein the first binding agent is biotin and the second binding agent is streptavidin or an analog thereof. ### Claim 9 (depends on 1) The method according to claim 1, wherein the solid support is part of a microfluidic chamber, said microfluidic chamber comprising: (i) said solid support; and (ii) a surface opposing the solid support, said surface having transverse structures that facilitate chaotic mixing of the fluid containing the cells or virus particles of interest when said fluid flows along said surface. ### Claim 10 (depends on 9) The method according to claim 9, wherein said transverse structures are surface ridges having a herringbone-structure. ### Claim 11 (depends on 1) The method according to claim 1, wherein step (c) is performed at a temperature of 28 to 40° C. ### Claim 12 A device for the isolation of cells or virus particles of interest expressing one or more predetermined surface marker(s), comprising: (a) a microfluidic chamber comprising: a solid support comprising an array of defined isolated spots of a solid support-bound binding agent; and (ii) a surface opposing the solid support, said surface having transverse structures that facilitate chaotic mixing of a fluid containing the cells or virus particles of interest when said fluid flows along said surface; and (b) a capillary micropipette. ### Claim 13 (depends on 12) The device according to claim 12, wherein said transverse structures are surface ridges having a herringbone-structure. ### Claim 14 (depends on 12) The device according to claim 12, wherein the binding agent is streptavidin or an analog thereof. ### Claim 15 (depends on 12) The device according to claim 12, further comprising means for heating the solid support. ### Claim 16 (depends on 1) The method according to claim 1, wherein step (c) is performed at a temperature of about 37° C.