# US 2018/0024045 A1 · Pipette Tip, Pipette, Apparatus and Kit for Light Measurement

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- Number: US 2018/0024045 A1
- Document ID: US20180024045A1
- Status: Published application
- Kind: A1
- Application number: 15/541,917
- Filed: 2016-01-08
- Published: 2018-01-25
- Earliest claimed: 2015-01-09
- Inventors: Brian Page
- Web page: https://patentgrep.com/patent/US20180024045A1

## CPC

- G01N 21/0303: {Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment (G01N21/031 - G01N21/15 take precedence)}
- B01L 3/021: {Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids}
- B01L 3/0275: {Interchangeable or disposable dispensing tips}
- B01L 3/508: {Rigid containers without fluid transport within}
- B01L 3/52: {Containers specially adapted for storing or dispensing a reagent (B01L3/02 takes precedence; storing or dispensing test elements G01N33/4875; automated reagent dispensing G01N35/1002)}
- G01N 21/03: Cuvette constructions
- G01N 21/255: {Details, e.g. use of specially adapted sources, lighting or optical systems}
- G01N 21/27: using photo-electric detection (G01N21/31 takes precedence){; circuits for computing concentration (logarithmic circuits G06G7/24; photometric circuits in general G01J)}
- B01L 2300/0654: Lenses; Optical fibres
- B01L 2300/0838: Capillaries
- G01N 2021/0325: {Cells for testing reactions, e.g. containing reagents}
- G01N 2021/0346: {Capillary cells; Microcells}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

## Abstract

A pipette tip comprises a vessel for attachment to a pipette, wherein the vessel is prefilled with a reagent or chemical to permit an assay to be carried out in the pipette tip itself. The pipette comprises a body portion for aspirating a fluid sample into the pipette tip when attached thereto, the body portion comprising at least one light source, or at least one entry point for light from at least one light source, providing an optical path of light that passes through the sample in a direction essentially along the longitudinal axis of the pipette tip.

## Claims

### Claim at position 1

1-15. (canceled)

### Claim 16

A pipette tip which comprises a vessel for attachment to a pipette, wherein the vessel is prefilled with a reagent or chemical to permit an assay to be carried out in the pipette tip itself and wherein the pipette comprises a body portion for aspirating a fluid sample into the pipette tip when attached thereto, the body portion comprising at least one light source, or at least one entry point for light from at least one light source, providing an optical path of light that passes through the sample in a direction essentially along the longitudinal axis of the pipette tip.

### Claim 17 (depends on 16)

A pipette tip according to claim 16 wherein the pipette tip has an opaque wall.

### Claim 18 (depends on 16)

A pipette tip according to claim 16 wherein the pipette tip is attached to the body portion.

### Claim 19

A method for measuring light output from a fluid sample and reagent, comprising providing a pipette tip or capillary tube having first and second open ends with the sample and reagent to be analysed contained therein, detecting light output from an open end of the pipette tip or capillary tube, wherein the sample is drawn into the pipette tip in a number of steps, and shining a light longitudinally through the pipette tip during the step process to obtain a calibration curve of the light detected.

### Claim 20 (depends on 19)

A method according to claim 19 wherein the number of steps is about 1 to about 10.

### Claim 21 (depends on 19)

A method according to claim 19 wherein the number of steps is about 4 or 5.

### Claim 22 (depends on 19)

A method according to claim 19, further comprising providing a light source, permitting light from the light source to enter one open end of the pipette tip or capillary tube, to pass through the sample contained therein, and to leave the pipette tip or capillary tube by its other open end for detection.

### Claim 23 (depends on 19)

A method according to claim 19 wherein the path of light passes through the sample in a direction essentially along the longitudinal axis of the pipette tip.

### Claim 24 (depends on 19)

A method according to claim 19 wherein the pipette tip is attached to a pipette body.

### Claim 25 (depends on 24)

A method according to claim 24, further comprising recharging the pipette body during sample detection and/or analysis.

### Claim 26 (depends on 19)

A method according to claim 19, further comprising controlling the intensity and/or wavelength of light input to the sample.

### Claim 27

A kit for light measurement, comprising a prefilled pipette tip or capillary tube for containing a fluid sample to be analysed, the pipette tip or capillary tube having first and second open ends, and light output guide means for guiding light output from at least one open end of the pipette tip or capillary tube to a photo detector.

### Claim 28 (depends on 27)

A kit according to claim 27, further comprising light input guide means for guiding light from a light source to one open end of the pipette tip or capillary tube so that the light passes through the sample and leaves by the other open end of the pipette tip or capillary tube.

### Claim 29 (depends on 16)

A kit for light measurement, comprising a pipette tip according to claim 16, and light output guide means for guiding light output from an open end of the pipette tip or capillary tube to a photo detector.