# US 2018/0023121 A1 · System and cartridge for efficient nucleic acid testing

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- Number: US 2018/0023121 A1
- Document ID: US20180023121A1
- Status: Published application
- Kind: A1
- Application number: 15/699,718
- Filed: 2017-09-08
- Published: 2018-01-25
- Earliest claimed: 2012-04-10
- Assignees: Keck Graduate Institute of Applied Life Sciences
- Applicants: Keck Graduate Institute of Applied Life Sciences
- Inventors: Angelika Niemz; Anna Hickerson; Kristina Roskos; Hsiang-Wei Lu
- Web page: https://patentgrep.com/patent/US20180023121A1

## CPC

- C12Q 1/686: Polymerase chain reaction [PCR]
- B01L 3/50273: {characterised by the means or forces applied to move the fluids}
- F04B 17/00: Pumps characterised by combination with, or adaptation to, specific driving engines or motors
- B01L 3/502723: {characterised by venting arrangements}
- B01L 7/00: Heating or cooling apparatus (autoclaves B01J3/04); Heat insulating devices
- B01L 2300/0663: Whole sensors
- B01L 2400/0421: electrophoretic flow
- B01L 2400/046: Chemical or electrochemical formation of bubbles
- B01L 2400/0481: squeezing of channels or chambers
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- F04B: POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS (machines for liquids, or pumps, of rotary-piston or oscillating-piston type F04C; non-positive-displacement pumps F04D; pumping of fluid by direct contact of another fluid or by using inertia of fluid to be pumped F04F)

## Abstract

A disposable and inexpensive biological diagnostic cartridge for the amplification and detection of nucleic acids includes a configuration having a reaction pouch for amplification which is compressed by a flexible pump pouch for detection of the amplified reaction.

## Claims

### Claim 1

A biological diagnostic cartridge comprising:
an inlet;
a flexible reaction pouch downstream of the inlet;
a flexible pump pouch adapted to compress the flexible reaction pouch; and
a detection chamber downstream of the flexible reaction pouch.

### Claim 2 (depends on 1)

The cartridge of claim 1, further comprising:
a first fluid path from the inlet to the flexible reaction pouch, and
a second fluid path from the flexible reaction pouch to the detection chamber.

### Claim 3 (depends on 2)

The cartridge of claim 2, wherein each of the first and second fluid paths is one-directional.

### Claim 4 (depends on 1)

The cartridge of claim 1, further comprising a pump adapted to inflate the flexible pump pouch.

### Claim 5 (depends on 4)

The cartridge of claim 4, further comprising a housing.

### Claim 6 (depends on 5)

The cartridge of claim 5, further comprising
a housing; and
a pump adapted to inflate the flexible pump pouch, wherein the housing comprises a port for receiving the pump.

### Claim 7 (depends on 6)

The cartridge of claim 6, wherein the pump comprises an electrolytic pump adapted to generate gas.

### Claim 8 (depends on 1)

The cartridge of claim 1, further comprising an overflow pool adjacent to the detection chamber.

### Claim 9 (depends on 1)

The cartridge of claim 1, wherein the pump pouch overlays the flexible reaction pouch.

### Claim 10 (depends on 1)

The cartridge of claim 1, further comprising a one-way valve downstream of the reaction pouch and upstream of the detection chamber.

### Claim 11 (depends on 1)

The cartridge of claim 1, further comprising at least a flow strip situated in the detection chamber.

### Claim 12 (depends on 1)

The cartridge of claim 1 further comprising a vent downstream of the inlet and upstream of the reaction pouch.

### Claim 13 (depends on 12)

The cartridge of claim 12, wherein the vent comprises a hydrophobic barrier.

### Claim 14 (depends on 1)

The cartridge of claim 1, wherein the pump pouch contains a fluid.

### Claim 15 (depends on 1)

The cartridge of claim 1, further comprising a second inlet, a second flexible reaction pouch downstream of the second inlet, a second flexible pump pouch adapted to compress the second flexible reaction pouch, a second detection chamber downstream of the second flexible reaction pouch.

### Claim 16 (depends on 15)

The cartridge of claim 15, further comprising a second flow strip.

### Claim 17 (depends on 16)

The cartridge of claim 16, wherein the second flow strip is arranged antiparallel or parallel to a first flow strip.