# US 2018/0022830 A1 · Peptides having affinity for polydimethylsiloxane, and uses thereof > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2018/0022830 A1 - Document ID: US20180022830A1 - Status: Published application - Kind: A1 - Application number: 15/550,137 - Filed: 2016-02-12 - Published: 2018-01-25 - Earliest claimed: 2015-02-13 - Assignees: National University Corporation Kyoto Institute of Technology; Nissan Chemical Industries, Ltd. - Applicants: National University Corporation Kyoto Institute of Technology; Nissan Chemical Industries, Ltd. - Inventors: Yoichi KUMADA; Ryoko OTSUKI; Ryota AKAI; Junko KATAYAMA; Kazutaka MATOBA - Web page: https://patentgrep.com/patent/US20180022830A1 ## CPC - C07K 17/08: the carrier being a synthetic polymer - C12N 9/88: Lyases (4.) - C07K 14/00: Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof - C07K 14/245: Escherichia (G) - C07K 14/4737: {C-reactive protein} - C12N 7/08: {Inactivation or attenuation} by serial passage of virus - C12N 9/00: Enzymes; Proenzymes; Compositions thereof (preparations containing enzymes for cleaning teeth A61K8/66, A61Q11/00; medicinal preparations containing enzymes or proenzymes A61K38/43; enzyme containing detergent compositions C11D; {enzymes with nucleic acid structure, e.g. ribozymes, C12N15/113}); Processes for preparing, activating, inhibiting, separating or purifying enzymes (preparation of malt C12C1/00) - C12N 15/00: Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor (mutants or genetically engineered microorganisms, per se C12N1/00, C12N5/00, C12N7/00; new plants per se A01H; plant reproduction by tissue culture techniques A01H4/00; new animals per se A01K67/00; use of medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases, gene therapy A61K48/00) - C12Y 401/99001: Tryptophanase (4.1.99.1) - C07K 2319/00: Fusion polypeptide - C07K 2319/21: containing a His-tag - C07K 2319/23: containing a GST-tag - C08G 77/04: Polysiloxanes - C12N 15/63: Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C12Y: ENZYMES - C08G: MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS ## Abstract An object of the present invention is to provide a polydimethylsiloxane substrate to which a peptide having an affinity for polydimethylsiloxane (PDMS) is bound; a method for immobilizing a target protein on a polydimethylsiloxane substrate; a peptide having an affinity for PDMS; a polynucleotide encoding a peptide having an affinity for PDMS; and a vector using thereof. A polydimethylsiloxane substrate to which a peptide having an affinity for polydimethylsiloxane is bound, the peptide comprising the following peptide (1a) or (1b), or a fragment thereof: (1a) a peptide comprising an amino acid sequence represented by at least one member selected from the group consisting of SEQ ID NOs: 1 to 9; and (1b) a peptide comprising the amino acid sequence of (1a) in which one or more amino acids are deleted, substituted, and/or added, the peptide having an affinity for polydimethylsiloxane. ## Claims ### Claim 1 A polydimethylsiloxane substrate to which a peptide having an affinity for polydimethylsiloxane is bound, the peptide comprising the following peptide (1a) or (1b), or a fragment thereof: (1a) a peptide comprising an amino acid sequence represented by at least one member selected from the group consisting of SEQ ID NOs: 1 to 9; and (1b) a peptide comprising the amino acid sequence of (1a) in which one or more amino acids are deleted, substituted, and/or added, the peptide having an affinity for polydimethylsiloxane. ### Claim 2 (depends on 1) The polydimethylsiloxane substrate according to claim 1, wherein the fragment comprises 15 to 58 amino acid residues. ### Claim 3 (depends on 1, 2) The polydimethylsiloxane substrate according to claim 1 or 2, on which a target protein is immobilized via the peptide having an affinity for polydimethylsiloxane. ### Claim 4 A method for immobilizing a target protein on a polydimethylsiloxane substrate, the method comprising the step of bringing a peptide having an affinity for polydimethylsiloxane into contact with the polydimethylsiloxane substrate, wherein the peptide is incorporated in the target protein, and the peptide comprises the following peptide (1a) or (1b), or a fragment thereof: (1a) a peptide comprising an amino acid sequence represented by at least one member selected from the group consisting of SEQ ID NOs: 1 to 9; and (1b) a peptide comprising the amino acid sequence of (1a) in which one or more amino acids are deleted, substituted, and/or added, the peptide having an affinity for polydimethylsiloxane. ### Claim 5 (depends on 1) A method for immobilizing a target protein on a polydimethylsiloxane substrate, the method comprising the step of binding the peptide having an affinity for polydimethylsiloxane bound to the polydimethylsiloxane substrate according to claim 1 to the target protein. ### Claim 6 A composition for immobilizing a target protein on a polydimethylsiloxane substrate, the composition comprising a peptide having an affinity for polydimethylsiloxane, the peptide comprising the following peptide (1a) or (1b), or a fragment thereof: (1a) a peptide comprising an amino acid sequence represented by at least one member selected from the group consisting of SEQ ID NOs: 1 to 9; and (1b) a peptide comprising the amino acid sequence of (1a) in which one or more amino acids are deleted, substituted, and/or added, the peptide having an affinity for polydimethylsiloxane. ### Claim 7 (canceled) ### Claim 8 A peptide having an affinity for polydimethylsiloxane, the peptide comprising the following peptide (1c) or (1d), or a fragment thereof: (1c) a peptide comprising an amino acid sequence represented by at least one member selected from the group consisting of SEQ ID NOs: 2 to 9; and (1d) a peptide comprising an amino acid sequence represented by at least one member selected from the group consisting of SEQ ID NOs: 1 to 9 in which one or more amino acids are deleted, substituted, and/or added, the peptide having an affinity for polydimethylsiloxane. ### Claim 9 (depends on 8) The peptide having an affinity for polydimethylsiloxane according to claim 8, wherein the fragment comprises 15 to 58 amino acid residues. ### Claim 10 (depends on 8) A polynucleotide encoding the peptide of claim 8. ### Claim 11 (depends on 10) The polynucleotide according to claim 10, which is represented by at least one member selected from the group consisting of SEQ ID NOs: 10 to 18. ### Claim 12 (depends on 10) A vector for expressing a peptide having an affinity for polydimethylsiloxane, the vector comprising the polynucleotide according to claim 10. ### Claim 13 (depends on 10) A vector for expressing a peptide fusion protein, the peptide fusion protein comprising a target protein and a peptide having an affinity for polydimethylsiloxane, the peptide comprising the following peptide (1c) or (1d), or a fragment thereof: (1c) a peptide comprising an amino acid sequence represented by at least one member selected from the group consisting of SEQ ID NOs: 2 to 9; and (1d) a peptide comprising an amino acid sequence represented by at least one member selected from the group consisting of SEQ ID NOs: 1 to 9 in which one or more amino acids are deleted, substituted, and/or added, the peptide having an affinity for polydimethylsiloxane, wherein the vector comprises the polynucleotide according to claim 10 and a polynucleotide encoding the target protein, wherein the polynucleotide according to claim 10 is linked to the polynucleotide encoding the target protein. ### Claim 14 (depends on 13) A transformant obtainable by introducing the vector according to claim 13 into a host cell to transform the host cell. ### Claim 15 (depends on 14) A peptide fusion protein comprising a target protein and a peptide having an affinity for polydimethylsiloxane, the peptide comprising the following peptide (1c) or (1d), or a fragment thereof: (1c) a peptide comprising an amino acid sequence represented by at least one member selected from the group consisting of SEQ ID NOs: 2 to 9; and (1d) a peptide comprising an amino acid sequence represented by at least one member selected from the group consisting of SEQ ID NOs: 1 to 9 in which one or more amino acids are deleted, substituted, and/or added, the peptide having an affinity for polydimethylsiloxane, wherein the peptide fusion protein is obtainable from the transformant according to claim 14. ### Claim 16 (depends on 8) A linker for immobilizing a target protein on a polydimethylsiloxane substrate, the linker comprising the peptide having an affinity for polydimethylsiloxane according to claim 8.