# US 2018/0021392 A1 · Methods of modulating immune and inflammatory responses via administration of an algal biomass > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2018/0021392 A1 - Document ID: US20180021392A1 - Status: Published application - Kind: A1 - Application number: 15/550,749 - Filed: 2016-02-16 - Published: 2018-01-25 - Earliest claimed: 2015-02-16 - Assignees: ZIVO Bioscience, Inc. - Applicants: Zivo Bioscience, Inc. - Inventors: Andrew A. Dahl; Amy E. Steffek - Web page: https://patentgrep.com/patent/US20180021392A1 ## CPC - A61K 36/05: Chlorophycota or chlorophyta (green algae), e.g. Chlorella - A61K 9/0053: {Mouth and digestive tract, i.e. intraoral and peroral administration} - A61K 36/02: Algae - A61P 11/00: Drugs for disorders of the respiratory system - A61P 19/02: for joint disorders, e.g. arthritis, arthrosis - A61P 29/00: Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID] - A23V 2200/02: Antioxidant - A23V 2200/324: having an effect on the immune system - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS - A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION ## Abstract An algal biomass and supernatant derived from at least one species of algae exhibits anti-inflammatory and immune response modulating properties. Methods of reducing the symptoms of or treating a condition or disease in an animal, including bovine mastitis and Bovine Respiratory Disease Complex, and the pain and discomfort caused by osteoarthritis, injury or overexertion or muscle and connective tissue strains, comprises administering to the animal in need thereof an algal biomass or supernatant derived from at least one species of algae, or an extract, derivative or biologically active compound derived from the algae species, biomass or supernatant, or compositions thereof. ## Claims ### Claim 1 A method of reducing the symptoms of, and/or treating a condition or disease in an animal, said disease characterized by inflammation or a loss of function, said method comprising administering to the animal in need thereof a therapeutically effective amount of a composition comprising an algal biomass or supernatant, wherein said algal biomass or supernatant is derived from at least one species of algae. ### Claim 2 (depends on 1) The method of claim 1, wherein said at least one species of algae comprises a klebsormidium species of algae. ### Claim 3 (depends on 2) The method of claim 2, wherein said species of algae is selected from the group consisting of klebsormidium acidophilum, klebsormidium bilatum, klebsormidium crenulatum, klebsormidium dissectum, klebsormidium drouetii, klebsormidium elegans, klebsormidium flaccidum, klebsormidium fluitans, klebsormidium fragile, klebsormidium klebsii, klebsormidium lamellosum, klebsormidium montanum, klebsormidium mucosum, klebsormidium nitens, klebsormidium pseudostichococcus, klebsormidium scopulinum, klebsormidium sterile, klebsormidium subtile, klebsormidium subtilissimum, klebsormidium tribonematoideum and mixtures thereof. ### Claim 4 (depends on 3) The method of claim 3, wherein said species of algae is selected from the group consisting of klebsormidium nitens, klebsormidium flaccidum, and mixtures thereof. ### Claim 5 (depends on 1) The method of claim 1, wherein said composition is a sterile injectable liquid further comprising a sterile diluent to form said sterile injectable liquid from said supernatant. ### Claim 6 (depends on 1) The method of claim 1, wherein said composition is a paste further comprising a vehicle to form said paste from said algal biomass. ### Claim 7 A method of treating bovine mastitis in a dairy cow, said method comprising administering to the dairy cow in need of treatment thereof a therapeutically effective amount of a composition comprising an algal biomass or supernatant, wherein said algal biomass or supernatant is derived from at least one species of algae. ### Claim 8 (depends on 7) The method of claim 7, wherein said at least one species of algae comprises a klebsormidium species of algae. ### Claim 9 (depends on 8) The method of claim 8, wherein said species of algae is selected from the group consisting of klebsormidium acidophilum, klebsormidium bilatum, klebsormidium crenulatum, klebsormidium dissectum, klebsormidium drouetii, klebsormidium elegans, klebsormidium flaccidum, klebsormidium fluitans, klebsormidium fragile, klebsormidium klebsii, klebsormidium lamellosum, klebsormidium montanum, klebsormidium mucosum, klebsormidium nitens, klebsormidium pseudostichococcus, klebsormidium scopulinum, klebsormidium sterile, klebsormidium subtile, klebsormidium subtilissimum, klebsormidium tribonematoideum and mixtures thereof. ### Claim 10 (depends on 9) The method of claim 9, wherein said species of algae is selected from the group consisting of klebsormidium nitens, klebsormidium flaccidum, and mixtures thereof. ### Claim 11 (depends on 7) The method of claim 7, wherein said composition is a sterile injectable liquid further comprising a sterile diluent to form said sterile injectable liquid from said supernatant. ### Claim 12 (depends on 7) The method of claim 7, wherein said composition is a paste further comprising a vehicle to form said paste from said algal biomass. ### Claim 13 7. A method of treating bovine respiratory disease complex in a beef or dairy cow, said method comprising administering to the animal in need of treatment thereof a therapeutically effective amount of a composition comprising an algal biomass or supernatant, wherein said algal biomass or supernatant is derived from at least one species of algae. ### Claim 14 (depends on 13) The method of claim 13, wherein said at least one species of algae comprises a klebsormidium species of algae. ### Claim 15 (depends on 14) The method of claim 14, wherein said species of algae is selected from the group consisting of klebsormidium acidophilum, klebsormidium bilatum, klebsormidium crenulatum, klebsormidium dissectum, klebsormidium drouetii, klebsormidium elegans, klebsormidium flaccidum, klebsormidium fluitans, klebsormidium fragile, klebsormidium klebsii, klebsormidium lamellosum, klebsormidium montanum, klebsormidium mucosum, klebsormidium nitens, klebsormidium pseudostichococcus, klebsormidium scopulinum, klebsormidium sterile, klebsormidium subtile, klebsormidium subtilissimum, klebsormidium tribonematoideum and mixtures thereof. ### Claim 16 (depends on 15) The method of claim 15, wherein said species of algae is selected from the group consisting of klebsormidium nitens, klebsormidium flaccidum, and mixtures thereof. ### Claim 17 (depends on 13) The method of claim 13, wherein said composition is a sterile injectable liquid further comprising a sterile diluent to form said sterile injectable liquid from said supernatant. ### Claim 18 (depends on 13) The method of claim 13, wherein said composition is a paste further comprising a vehicle to form said paste from said algal biomass. ### Claim 19 A method of producing an algal biomass having anti-inflammatory and/or immune response modulating properties, said method comprising obtaining a sample of NCMA Deposit # PATENT201602001 and drying said sample. ### Claim 20 A method of producing a supernatant having anti-inflammatory and/or immune response modulating properties, said method comprising obtaining a sample of NCMA Deposit # PATENT201602001 and mechanically separating said supernatant from said sample. ### Claim 21 An anti-inflammatory and immune response modulating composition comprising an algal biomass or a supernatant derived from at least one species of algae. ### Claim 22 (depends on 21) The composition of claim 21, wherein said at least one species of algae comprises a klebsormidium species of algae. ### Claim 23 (depends on 22) The composition of claim 22, wherein said species of algae is selected from the group consisting of klebsormidium acidophilum, klebsormidium bilatum, klebsormidium crenulatum, klebsormidium dissectum, klebsormidium drouetii, klebsormidium elegans, klebsormidium flaccidum, klebsormidium fluitans, klebsormidium fragile, klebsormidium klebsii, klebsormidium lamellosum, klebsormidium montanum, klebsormidium mucosum, klebsormidium nitens, klebsormidium pseudostichococcus, klebsormidium scopulinum, klebsormidium sterile, klebsormidium subtile, klebsormidium subtilissimum, klebsormidium tribonematoideum and mixtures thereof. ### Claim 24 (depends on 23) The composition of claim 23, wherein said species of algae is selected from the group consisting of klebsormidium nitens, klebsormidium flaccidum, and mixtures thereof. ### Claim 25 (depends on 21) The composition of claim 21, wherein said algal biomass or said supematant is obtained by processing the biological material of NCMA Deposit # PATENT201602001.