# US 2018/0021384 A1 · Method of Tissue Repair

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- Number: US 2018/0021384 A1
- Document ID: US20180021384A1
- Status: Published application
- Kind: A1
- Application number: 15/697,046
- Filed: 2017-09-06
- Published: 2018-01-25
- Earliest claimed: 2009-03-27
- Assignees: Orthocell Pty Ltd
- Applicants: Orthocell Pty Ltd
- Inventors: Ming Hao Zheng
- Web page: https://patentgrep.com/patent/US20180021384A1

## CPC

- A61K 35/32: Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
- A61L 15/60: Liquid-swellable gel-forming materials, e.g. super-absorbents
- A61P 19/04: for non-specific disorders of the connective tissue
- A61P 21/00: Drugs for disorders of the muscular or neuromuscular system
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

The present invention relates to methods of repairing tissue. More specifically, the present invention relates to methods of using cells and an implantable support for the repair of tissue defects, where the implantable support and cells are implanted into the tissue defect less than 2 hours after the cells are applied to the support.

## Claims

### Claim 1

An in vivo method of repairing connective tissue in a mammalian_subject in need thereof comprising the steps of:
(a) providing an implantable support comprising a collagen-containing membrane and a sample of autologous chondrocyte cells;
(b) heating the implantable support to between 35° C. and 37° C.;
(c) applying said sample of cells to the surface of said support to produce an implantable matrix and incubating said implantable matrix for between 15 minutes to 1 hour and 50 minutes such that at least 90% of said cells adhere to the surface of the matrix; and
(d) implanting said matrix into or adjacent to said connective tissue in need of repair.

### Claim 2 (depends on 1)

The method of claim 1, wherein the connective tissue is selected from the group consisting of cartilage, bone, and tendon.

### Claim 3 (depends on 1)

The method of claim 1, wherein the mammalian subject is a horse or a human.

### Claim 4 (depends on 3)

The method of claim 3, wherein the mammalian subject is a human.

### Claim 5 (depends on 1)

The method of claim 1, wherein the matrix is implanted between 15 minutes and about 1 hour 30 minutes after the cells have been applied to the support.

### Claim 6 (depends on 1)

The method of claim 1, wherein the matrix is implanted about 20 minutes after the cells have been applied to the support.

### Claim 7 (depends on 1)

The method of claim 1, wherein the matrix is implanted between 30 minutes and about 1 hour and 10 minutes after the cells have been applied to the membrane.

### Claim 8 (depends on 1)

The method of claim 1, wherein the matrix is implanted about 40 minutes after the cells have been applied to the support.

### Claim 9 (depends on 1)

The method of claim 1, further comprising step (e) coating the matrix with a cell sealant prior to implantation.

### Claim 10 (depends on 9)

The method of claim 9, wherein the cell sealant is a fibrin sealant.

### Claim 11

An in vivo method of repairing connective tissue in a mammalian subject in need thereof comprising the steps of:
(a) isolating chondrocyte cells from said mammalian subject;
(b) culturing said isolated chondrocyte cells to produce cultured chondrocyte cells;
(c) harvesting said cultured chondrocyte cells to provide a sample of about 106 cultured chondrocyte cells suspended in said subject's own serum;
(d) providing an implantable support comprising a collagen-containing membrane;
(e) heating the implantable support to between 35° C. and 3° C.;
(f) applying said sample of about 106 cultured chondrocyte cells to the surface of said support to produce an implantable matrix and incubating said implantable matrix for between 15 minutes to 1 hour and 50 minutes such that at least 90% of said chondrocyte cells adhere to the surface of the matrix; and
(g) implanting said matrix into or adjacent to said connective tissue in need of repair.