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US 2018/0021285 A1Published application
Method for visualization of calcium containing components in biological systems
AssigneeVisikol, Inc.
InventorsNicholas Ingram Crider, Thomas Steven Villani, JR., Michael Thomas Johnson
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Abstract
Provided are compositions and methods for visualizing the skeletal tissues of a biological specimen, comprising treating the specimen with a calcium chelating agent, a staining solution, and a clearing solution.
Claims22
A method of visualizing a calcified component in a tissue specimen, comprising:
- a) contacting a tissue specimen with a chelating agent, the chelating agent binding to the calcified component;
- b) staining the contacted tissue specimen with a calcium binding dye solution;
- and
- c) clarifying the stained specimen with a clearing solution that makes visible the calcified components.
1 more element
A kit for visualizing a calcified component in a tissue specimen, comprising:
- a chelating agent;
- a calcium-binding dye;
- and
- a clearing solution.
1 more element
Dependencies are inferred from the claim text.