# US 2018/0020688 A1 · Nisin Resistant Strains of Lactobacilli and Methods for Reducing the Post Acidification in Food Products

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- Number: US 2018/0020688 A1
- Document ID: US20180020688A1
- Status: Published application
- Kind: A1
- Application number: 15/653,590
- Filed: 2017-07-19
- Published: 2018-01-25
- Earliest claimed: 2013-12-04
- Assignees: Compagnie Gervais Danone
- Applicants: Compagnie Gervais Danone
- Inventors: Anne Druesne; Peggy Garault; Jean-Michel Faurie
- Web page: https://patentgrep.com/patent/US20180020688A1

## CPC

- A23C 9/123: using only microorganisms of the genus lactobacteriaceae; Yoghurt (A23C9/13 takes precedence)
- A23C 9/1238: {using specific L. bulgaricus or S. thermophilus microorganisms; using entrapped or encapsulated yoghurt bacteria; Physical or chemical treatment of L. bulgaricus or S. thermophilus cultures; Fermentation only with L. bulgaricus or only with S. thermophilus}
- C12N 1/205: Bacterial isolates
- A23V 2400/123: Bulgaricus
- A23V 2400/147: Helveticus
- C12R 2001/225: Lactobacillus
- A23C: DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF (preservation thereof A23B11/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

The invention relates to nisin-resistant lactobacilli and their use for preparing food products with reduced post-acidification.

## Claims

### Claim at position 1

1-22. (canceled)

### Claim 23

A mutant strain of Lactobacillus bulgaricus selected from the group consisting of the L. bulgaricus strain deposited as CNCM Deposit Number I-4452, CNCM Deposit Number I-4453 and CNCM Deposit Number I-4454.

### Claim 24 (depends on 23)

A fermented dairy product comprising the mutant strain of claim 23, wherein the mutant strain is the L. bulgaricus strain deposited as CNCM Deposit Number I-4452.

### Claim 25 (depends on 23)

A fermented dairy product comprising the mutant strain of claim 23, wherein the mutant strain is the L. bulgaricus strain deposited as CNCM Deposit Number I-4453.

### Claim 26 (depends on 23)

A fermented dairy product comprising the mutant strain of claim 23, wherein the mutant strain is the L. bulgaricus strain deposited as CNCM Deposit Number I-4454.

### Claim 27 (depends on 23)

A method of preserving a dairy product comprising administering the mutant strain of claim 23 to dairy product.

### Claim 28 (depends on 27)

The method of claim 27, wherein the mutant strain in contained in a food matrix when it is administered to the dairy product.

### Claim 29 (depends on 27)

The method of claim 27, wherein the dairy product is a fermented dairy product and the mutant strain is administered the dairy product either before, during or after fermentation of the dairy product.

### Claim 30 (depends on 29)

The method of claim 29, further comprising storing the fermented dairy product at a temperature of between about 0° C. and 25° C. after administration of the mutant strain.

### Claim 31 (depends on 29)

The method of claim 29, wherein the fermentation is carried out in the presence of at least one additional living bacterium.

### Claim 32 (depends on 31)

The method of claim 31, wherein the at least one additional living bacterium is selected from the group consisting of Streptococcus spp. and Lactobacillus spp.