# US 2018/0020671 A1 · Agriculturally beneficial microbes, microbial compositions, and consortia > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2018/0020671 A1 - Document ID: US20180020671A1 - Status: Published application - Kind: A1 - Application number: 15/549,815 - Filed: 2016-02-09 - Published: 2018-01-25 - Earliest claimed: 2015-02-09 - Assignees: Bioconsortia, Inc. - Applicants: Bioconsortia, Inc. - Inventors: Peter WIGLEY; Susan TURNER; Caroline GEORGE; Thomas WILLIAMS; Kelly ROBERTS; Graham HYMUS; Kelvin LAU - Web page: https://patentgrep.com/patent/US20180020671A1 ## CPC - A01N 63/20: Bacteria; Substances produced thereby or obtained therefrom - C12N 1/20: Bacteria; Culture media therefor - C12N 1/205: Bacterial isolates - C12R 2001/01: Bacteria or Actinomycetales {; using bacteria or Actinomycetales} - C12R 2001/07: Bacillus - C12R 2001/38: Pseudomonas - C12R 2001/41: Rhizobium - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS ## Abstract The disclosure relates to isolated microorganisms-including novel strains of the microorganisms-microbial consortia, and agricultural compositions comprising the same. Furthermore, the disclosure teaches methods of utilizing the described microorganisms, microbial consortia, and agricultural compositions comprising the same, in methods for imparting beneficial properties to target plant species. In particular aspects, the disclosure provides methods of increasing desirable plant traits in agronomically important crop species. ## Claims ### Claim at position 1 1-77. (canceled) ### Claim 78 A method of imparting at least one beneficial trait upon a plant species, the method comprising: applying at least one isolated bacterial species to said plant, or to a growth medium in which said plant is located, wherein at least one isolated bacterial species is selected from the group consisting of Acidovorax soli, Agrobacterium fabrum, Arthrobacter cupressi, Bosea eneae, Bosea minatitlanensis, Bosea robinae, Caulobacter henricii, Chitinophaga arvensicola, Chitinophaga terrae, Delftia lacustris, Ensifer adhaerens, Duganella radicis, Duganella violaceinigra (Pseudoduganella violaceinigra), Dyadobacter soli, Flavobacterium glaciei, Frateuria aurantia, Frateuria terrea, Herbaspirillum chlorophenolicum, Janthinobacterium agaricidamnosum, Janthinobacterium lividum, Luteibacter yeojuensis, Massilia albidiflava, Massilia niastensis, Microbacterium olievorans, Novosphingobium lindaniclasticum, Novosphingobium resinovorum, Novosphingobium rosa, Paenibacillus amylolyticus, Paenibacillus chondroitinus, Paenibacillus glycanilyticus, Pedobacter rhizosphaerae (Pedobacter soli), Pedobacter terrae, Polaromonas ginsengisoli, Pseudomonas jinjuensis, Ramlibacter henchirensis, Rhizobium pusense, Rhizobium rhizoryzae, Rhodoferax ferrireducens, Sinorhizobium chiapanecum, Sphingobium quisquiliarum, Sphingopyxis alaskensis, Stenotrophomonas terrae, Variovorax ginsengisoli and combinations thereof. ### Claim 79 (depends on 78) The method of claim 78, wherein the applying comprises applying a microbial consortia comprising at least two of the isolated bacterial species. ### Claim 80 (depends on 78) The method of claim 78, wherein the isolated bacterial species is formulated in an agricultural composition with one or more of the following: an agriculturally acceptable carrier, a pesticide, a plant growth regulator, a beneficial agent, and a biologically active agent. ### Claim 81 (depends on 80) The method of claim 80, wherein the isolated bacterial species is present in the agricultural composition at 1×103to 1×1012 bacterial cells per gram. ### Claim 82 (depends on 78) The method of claim 78, wherein the applying occurs by: coating a plant seed with said bacteria, coating a plant part with said bacteria, spraying said bacteria onto a plant part, spraying said bacteria into a furrow into which a plant or seed will be placed, drenching said bacteria onto a plant part or into an area into which a plant will be placed, spreading said bacteria onto a plant part or into an area into which a plant will be placed, broadcasting said bacteria onto a plant part or into an area into which a plant will be placed, combining the bacteria with a fertilizer or other agricultural composition and combinations thereof. ### Claim 83 (depends on 82) The method of claim 82, wherein the seed coating comprises at least one isolated bacterial species at a concentration of 1×105to 1×109 bacterial cells per seed. ### Claim 84 A method of imparting at least one beneficial trait upon a plant species, the method comprising: applying at least one isolated bacterial strain to a plant or to a growth medium having a plant, wherein the at least one bacterial species is selected from the group consisting of: a) Acidovorax soli deposited as NRRL Accession Deposit No. NRRL B-67181; b) Acidovorax soli deposited as NRRL Accession Deposit No. NRRL B-67182; c) Arthrobacter cupressi deposited as NRRL Accession Deposit No. NRRL B-67183; d) Arthrobacter cupressi deposited as NRRL Accession Deposit No. NRRL B-67184; e) Bosea eneae deposited as NRRL Accession Deposit No. NRRL B-67185; f) Bosea robiniae deposited as NRRL Accession Deposit No. NRRL B-67186; g) Bosea thiooxidans deposited as NRRL Accession Deposit No. NRRL B-67187; h) Chitinophaga terrae deposited as NRRL Accession Deposit No. NRRL B-67188; i) Chitinophaga terrae deposited as NRRL Accession Deposit No. NRRL B-67189; j) Delftia lacustris deposited as NRRL Accession Deposit No. NRRL B-67190; k) Delftia lacustris deposited as NRRL Accession Deposit No. NRRL B-67191; l) Duganella radicis deposited as NRRL Accession Deposit No. NRRL B-67192; m) Duganella violaceinigria deposited as NRRL Accession Deposit No. NRRL B-67193; n) Dyadobacter soli deposited as NRRL Accession Deposit No. NRRL B-67194; o) Dyadobacter soli deposited as NRRL Accession Deposit No. NRRL B-67195; p) Flavobacterium glacei deposited as NRRL Accession Deposit No. NRRL B-67196; q) Herbaspirillum chlorophenolicum deposited as NRRL Accession Deposit No. NRRL B-67197; r) Massilia albidiflava deposited as NRRL Accession Deposit No. NRRL B-67198; s) Massilia niastensis deposited as NRRL Accession Deposit No. NRRL B-67199; t) Novosphingobium lindaniclasticum deposited as NRRL Accession Deposit No. NRRL B-67201; u) Novosphingobium lindaniclasticum deposited as NRRL Accession Deposit No. NRRL B-67200; v) Novosphingobium resinovorum deposited as NRRL Accession Deposit No. NRRL B-67202; w) Novosphingobium resinovorum deposited as NRRL Accession Deposit No. NRRL B-67203; x) Paenibacillus glycanilyticus deposited as NRRL Accession Deposit No. NRRL B-67204; y) Pedobacter soli deposited as NRRL Accession Deposit No. NRRL B-67205; z) Pedobacter terrae deposited as NRRL Accession Deposit No. NRRL B-67206; aa) Pseudomonas jinjuensis deposited as NRRL Accession Deposit No. NRRL B-67207; bb) Ramlibacter henchirensis deposited as NRRL Accession Deposit No. NRRL B-67208; cc) Ramlibacter henchirensis deposited as NRRL Accession Deposit No. NRRL B-67209; dd) Rhizobium rhizoryzae deposited as NRRL Accession Deposit No. NRRL B-67210; ee) Rhizobium rhizoryzae deposited as NRRL Accession Deposit No. NRRL B-67211; ff) Rhizobium sp. deposited as NRRL Accession Deposit No. NRRL B-67212; gg) Ensifer adhaerens deposited as NRRL Accession Deposit No. NRRL B-67213; hh) Sphingopyxis alaskensis deposited as NRRL Accession Deposit No. NRRL B-67214; ii) Sphingopyxis alaskensis deposited as NRRL Accession Deposit No. NRRL B-67215; jj) Variovorax ginsengisoli deposited as NRRL Accession Deposit No. NRRL B-67216; kk) Variovorax ginsengisoli deposited as NRRL Accession Deposit No. NRRL B-67217; ll) Achromobacter pulmonis deposited as NRRL Accession Deposit No. NRRL B-67174; mm) Chryseobacterium daecheongense deposited as NRRL Accession Deposit No. NRRL B-67172; nn) Duganella radicis deposited as NRRL Accession Deposit No. NRRL B-67166; oo) Exiguobacterium aurantiacum deposited as NRRL Accession Deposit No. NRRL B-67175; pp) Exiguobacterium sibiricum deposited as NRRL Accession Deposit No. NRRL B-67167; qq) Kosakonia radicincitans deposited as NRRL Accession Deposit No. NRRL B-67171; rr) Microbacterium oleivorans deposited as NRRL Accession Deposit No. NRRL B-67170; ss) Novosphingobium sediminicola deposited as NRRL Accession Deposit No. NRRL B-67168; tt) Pedobacter terrae deposited as NRRL Accession Deposit No. NRRL B-67176; uu) Rahnella aquatilis deposited as NRRL Accession Deposit No. NRRL B-67165; vv) Agrobacterium fabrum deposited as NRRL Accession Deposit No. NRRL B-67173; ww) Ensifer adhaerens deposited as NRRL Accession Deposit No. NRRL B-67169; xx) Pantoea vagans deposited as NRRL Accession Deposit No. NRRL B-67224; yy) Pseudomonas oryzihabitans deposited as NRRL Accession Deposit No. NRRL B-67225; zz) Stenotrophomonas maltophilia deposited as NRRL Accession Deposit No. NRRL B-67226; aaa) Rahnella aquatilis deposited as NRRL Accession Deposit No. NRRL B-67229; bbb) Rahnella aquatilis deposited as NRRL Accession Deposit No. NRRL B-67228; ccc) Rhodococcus erythropolis deposited as NRRL Accession Deposit No. NRRL B-67227; ddd) Herbaspirillum chlorophenolicum deposited as NRRL Accession Deposit No. NRRL B-67236; eee) Bacillus niacini deposited as NRRL Accession Deposit No. NRRL B-67230; fff) Polaromonas ginsengisoli deposited as NRRL Accession Deposit No. NRRL B-67231; ggg) Polaromonas ginsengisoli deposited as NRRL Accession Deposit No. NRRL B-67234; hhh) Duganella violaceinigra deposited as NRRL Accession Deposit No. NRRL B-67232; iii) Duganella violaceinigra deposited as NRRL Accession Deposit No. NRRL B-67233; and jjj) Masilia niastensis deposited as NRRL Accession Deposit No. NRRL B-67235. ### Claim 85 (depends on 84) The method of claim 84, wherein the applying comprises applying a microbial consortia comprising at least two of the isolated bacterial strains. ### Claim 86 (depends on 84) The method of claim 84, wherein the applying occurs by: coating a plant seed with said bacteria, coating a plant part with said bacteria, spraying said bacteria onto a plant part, spraying said bacteria into a furrow into which a plant or seed will be placed, drenching said bacteria onto a plant part or into an area into which a plant will be placed, spreading said bacteria onto a plant part or into an area into which a plant will be placed, broadcasting said bacteria onto a plant part or into an area into which a plant will be placed, combining the bacteria with a fertilizer or other agricultural composition and combinations thereof. ### Claim 87 (depends on 84) The method of claim 84, wherein the isolated bacterial strain is formulated in an agricultural composition with one or more of the following: an agriculturally acceptable carrier, a pesticide, a plant growth regulator, a beneficial agent or a biologically active agent. ### Claim 88 An agricultural composition, comprising: a) at least one isolated bacterial species selected from the group consisting of Acidovorax soli, Agrobacterium fabrum, Arthrobacter cupressi, Bosea eneae, Bosea minatitlanensis, Bosea robinae, Caulobacter henricii, Chitinophaga arvensicola, Chitinophaga terrae, Delftia lacustris, Ensifer adhaerens, Duganella radicis, Duganella violaceinigra (Pseudoduganella violaceinigra), Dyadobacter soli, Flavobacterium glaciei, Frateuria aurantia, Frateuria terrea, Herbaspirillum chlorophenolicum, Janthinobacterium agaricidamnosum, Janthinobacterium lividum, Luteibacter yeojuensis, Massilia albidiflava, Massilia niastensis, Microbacterium olievorans, Novosphingobium lindaniclasticum, Novosphingobium resinovorum, Novosphingobium rosa, Paenibacillus amylolyticus, Paenibacillus chondroitinus, Paenibacillus glycanilyticus, Pedobacter rhizosphaerae (Pedobacter soli), Pedobacter terrae, Polaromonas ginsengisoli, Pseudomonas jinjuensis, Ramlibacter henchirensis, Rhizobium pusense, Rhizobium rhizoryzae, Rhodoferax ferrireducens, Sinorhizobium chiapanecum, Sphingobium quisquiliarum, Sphingopyxis alaskensis, Stenotrophomonas terrae, Variovorax ginsengisoli, and combinations thereof; and b) an agriculturally acceptable carrier, a pesticide, a plant growth regulator, a beneficial agent and/or a biologically active agent. ### Claim 89 (depends on 88) The agricultural composition according to claim 88, wherein said agricultural composition is formulated as a seed coating, a fertilizer additive, a spray, a drench, and combinations thereof. ### Claim 90 A microbial consortia, comprising at least two microbes selected from the group consisting of Acidovorax soli, Agrobacterium fabrum, Arthrobacter cupressi, Bosea eneae, Bosea minatitlanensis, Bosea robinae, Caulobacter henricii, Chitinophaga arvensicola, Chitinophaga terrae, Delftia lacustris, Ensifer adhaerens, Duganella radicis, Duganella violaceinigra (Pseudoduganella violaceinigra), Dyadobacter soli, Flavobacterium glaciei, Frateuria aurantia, Frateuria terrea, Herbaspirillum chlorophenolicum, Janthinobacterium agaricidamnosum, Janthinobacterium lividum, Luteibacter yeojuensis, Massilia albidiflava, Massilia niastensis, Microbacterium olievorans, Novosphingobium lindaniclasticum, Novosphingobium resinovorum, Novosphingobium rosa, Paenibacillus amylolyticus, Paenibacillus chondroitinus, Paenibacillus glycanilyticus, Pedobacter rhizosphaerae (Pedobacter soli), Pedobacter terrae, Polaromonas ginsengisoli, Pseudomonas jinjuensis, Ramlibacter henchirensis, Rhizobium pusense, Rhizobium rhizoryzae, Rhodoferax ferrireducens, Sinorhizobium chiapanecum, Sphingobium quisquiliarum, Sphingopyxis alaskensis, Stenotrophomonas terrae, Variovorax ginsengisoli, microbes listed in table 1, microbes listed in table 2, microbes listed in table 3, and combinations thereof. ### Claim 91 (depends on 90) A microbial consortia having substantially similar genetic characteristics as a microbial consortia according to claim 90. ### Claim 92 (depends on 90) The microbial consortia of claim 90, wherein at least one of the microbes is mutated. ### Claim 93 (depends on 90) A cell-free or inactivated preparation of the microbial consortia according to claim 90. ### Claim 94 (depends on 90) A metabolite produced by the microbial consortia according to claim 90. ### Claim 95 (depends on 88) A synthetic combination of a plant and the agricultural composition of claim 88. ### Claim 96 (depends on 95) The synthetic combination of claim 95, comprising at least two of the isolated bacterial species, or different strains of the same species. ### Claim 97 (depends on 96) The synthetic combination of claim 96, wherein the at least two of the isolated bacterial species or different strains of the same species are coated onto the seed of the plant or applied onto the surface of a part of the plant, or applied into an area into which a plant will be planted, or combined with a fertilizer or other agricultural composition.