Methods and Systems for Image Data Processing

A system, comprising:

• an imaging subsystem configured to image, at different wavelength bands, particles disposed within the imaging subsystem, wherein the imaging comprises:
• selecting a first set of one or more optical filters corresponding to a first wavelength band;
• illuminating the particles through the first set of optical filters;
• selecting a second set of one or more optical filters corresponding to a second wavelength band;
• and
• illuminating the particles through the second set of optical filters;
• and
• a data processing subsystem configured to:
• store data acquired for multiple images of the particles, and wherein particular images of the multiple images include spots corresponding to the particles, wherein a first image of the particular images corresponds to the first set of optical filters, wherein a second image of the particular images corresponds to the second set of optical filters;
• create a first composite image of the multiple images, wherein the first composite image includes first composite spots corresponding to the particles, the first composite spots having a first amount of misalignment from the spots in the particular images;
• and
• modify coordinates of at least one of the multiple images such that a second composite image based on the modified coordinates includes second composite spots having a second, smaller amount of misalignment from the spots in the multiple images.

A method, comprising:

• a computing system receiving data corresponding to multiple images of a plurality of particles, wherein the multiple images correspond to different wavelength bands, wherein particular images of the multiple images include spots corresponding to individual ones of the plurality of particles, wherein a first image of the particular images corresponds to a first set of one or more optical filters, and wherein a second image of the particular images corresponds to a second set of one or more optical filters;
• the computing system creating a first composite image based on the multiple images, wherein the first composite image includes first composite spots corresponding to the particles, the first composite spots having a first amount of misalignment from the spots in the particular images;
• the computing system modifying coordinates of at least one of the multiple images such that a second composite image based on the modified coordinates includes second composite spots having a second, smaller amount of misalignment from the spots in the multiple images;
• and
• the computing system outputting the second composite image.

An article of manufacture including a computer-readable medium having instructions coded thereon that, in response to execution by a computing system, cause the computing system to carry out operations comprising:

• acquiring data corresponding to multiple images of a plurality of particles, wherein the multiple images correspond to different wavelength bands, wherein particular images of the multiple images include spots corresponding to individual ones of the plurality of particles, wherein a first image of the particular images corresponds to a first set of one or more optical filters, and wherein a second image of the particular images corresponds to a second set of one or more optical filters;
• creating a first composite image based on the multiple images, wherein the first composite image includes first composite spots corresponding to the particles, the first composite spots having a first amount of misalignment from the spots in the particular images;
• and
• modifying coordinates of at least one of the multiple images such that a second composite image based on the modified coordinates includes second composite spots having a second, smaller amount of misalignment from the spots in the multiple images.