Systems and methods for cluster matching across samples and guided visualization of multidimensional cytometry data

A system for matching clusters in data corresponding to different samples, the system comprising:

• memory in communication with storage configured to store first sample data, second sample data, an identification of one or more clusters in the first sample data, and an identification of one or more clusters in the second sample data;
• and
• a processor comprising:
• an adaptive binning module configured to perform multivariate adaptive binning on a combined data set including the first sample data and the second sample data to determine a combined binning pattern;
• a dissimilarity module configured to, for at least some combinations of a first identified cluster in the first sample data and a second identified cluster in the second sample data, determine a dissimilarity score for the combination based on a quadratic form distance for multi-dimensional data using the combined binning pattern applied to the first identified cluster in the first data set and the combined binning pattern applied to the second identified cluster in the second data set;
• a matching and merging candidate identification module configured to identify one or more matched clusters and one or more merging candidates based on comparisons of the determined dissimilarity scores for the combinations;
• and
• a determination module configured to determine, for each merging candidate in the first sample data or the second sample data, whether the merging candidate corresponds to a split of a cluster in the other sample data or corresponds to a missing cluster in the other sample data.

A method comprising:

• rendering a graphical user interface, the graphical user interface comprising:
• a first interactive display including a two-dimensional plot of at least portion of a data set, the data set including data corresponding to measurements of a plurality of parameters for a plurality of items, the data set having a plurality of dimensions, each dimension corresponding to measurements of a parameter in the plurality of parameters for the plurality of items, wherein the two-dimensional plot displays data corresponding to measurements of a first parameter for a second plurality of items and data corresponding to measurements of a second parameter for the second plurality of items, the second plurality of items being at least a subset of the first plurality of items;
• and
• a second interactive display including a plurality of single parameters charts or graphs each single parameter chart or graph displaying information corresponding to one-dimensional measurements of a different parameter for a third plurality of items that includes some or all of the second plurality of items.