Nucleic acid constructs, plants comprising same and uses thereof in enhancing plant pest resistance and altering plant monoterpene profile
AssigneeFutragene Israel Ltd.
InventorsDror AVISAR, Miron ABRAMSON, Tany SINAI, Roee SHAVIT
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Abstract
Nucleic acid constructs encoding Geraniol Synthase (GS), Geraniol Reductase (GR), Geraniol Dehydrogenase (GD), and/or Citral Reductase (CR) and plants comprising same are provided.
Claims36
A genetically modified woody plant comprising a heterologous nucleic acid sequence encoding at least one polypeptide selected from the group consisting of Geraniol Synthase (GS), Geraniol Reductase (GR), Geraniol Dehydrogenase (GD) and Citral Reductase (CR).
A nucleic acid construct comprising:
- a nucleic acid sequence encoding at least two polypeptides selected from the group consisting of Geraniol Synthase (GS), Geraniol Reductase (GR), Geraniol Dehydrogenase (GD) and Citral Reductase (CR) and at least one cis-acting regulatory element for directing expression of said nucleic acid sequence;
- or
- a nucleic acid sequence encoding Geraniol Dehydrogenase (GD) and a cis-acting regulatory element for directing expression of said nucleic acid sequence in a plant cell;
- or
- a nucleic acid sequence encoding Citral Reductase (CR) and a cis-acting regulatory element for directing expression of said nucleic acid sequence in a plant cell, wherein said nucleic acid sequence further comprises a chloroplast leader peptide;
- or
- a nucleic acid sequence encoding Geraniol Reductase (GR) and a cis-acting regulatory element for directing expression of said nucleic acid sequence in a plant cell, wherein said nucleic acid sequence is devoid of a peroxisome C-terminus tri-amino acid signal (SRL) and comprises a chloroplast leader peptide.
A nucleic acid construct system comprising at least two nucleic acid constructs expressing at least two polypeptides selected from the group consisting of Geraniol Synthase (GS), Geraniol Reductase (GR), Geraniol Dehydrogenase (GD) and Citral Reductase (CR).
4-6. (canceled)
(canceled)
10-11. (canceled)
A method of enhancing resistance of a woody plant to pest infection, the method comprising expressing in the woody plant at least one recombinant polypeptide selected from the group consisting of Geraniol Synthase (GS), Geraniol Reductase (GR), Geraniol Dehydrogenase (GD) and Citral Reductase (CR), thereby enhancing the resistance of the woody plant to pest infection.
A method of enhancing at least one of geraniol, geranial, neral, citronellol, citronellal and citral oil content of a woody plant, the method comprising expressing in the woody plant at least one recombinant polypeptide selected from the group consisting of Geraniol Synthase (GS), Geraniol Reductase (GR), Geraniol Dehydrogenase (GD) and Citral Reductase (CR), thereby enhancing at least one of geraniol, geranial, neral, citronellol, citronellal and citral oil content of the woody plant.
An eucalyptus oil having an increased content of at least one monoterpene selected from the group consisting of geraniol, geranial, neral, citronellol, citronellal and citral;
- as compared to a eucalyptus oil of a non-genetically modified eucalyptus.
37-39. (canceled)
41-43. (canceled)
Dependencies are inferred from the claim text.