Method of increasing resistance against soybean rust in transgenic plants by increasing the scopoletin content

A recombinant vector construct comprising one or more of the nucleic acids selected from the group consisting of:

• (a) a nucleic acid encoding F6H1 protein wherein said F6H1 protein is encoded by
• (i) a nucleic acid having at least 70% identity, at least 80% identity, at least 90% identity, at least 95% identity with SEQ ID NO:
• 1 or a functional fragment thereof, or a splice variant thereof;
• (ii) a nucleic acid coding for a protein having at least 70% identity, at least 80% identity, at least 90% identity, at least 95% identity with SEQ ID NO:
• 2 or a functional fragment thereof;
• (iii) an exogenous nucleic acid capable of hybridizing under stringent conditions with a complementary sequence of any of the nucleic acids according to (i) or (ii);
• or
• (iv) a nucleic acid encoding the same F6H1 protein as the nucleic acids of (i) to (iii) above, but differing from the nucleic acids of (i) to (iii) above due to the degeneracy of the genetic code;
• operably linked with a promoter and a transcription termination sequence,
• (b) a nucleic acid encoding CCoAOMT1 protein wherein said CCoAOMT1 protein is encoded by
• (i) a nucleic acid having at least 70% identity, at least 80% identity, at least 90% identity, at least 95% identity with SEQ ID NO:
• 3 or a functional fragment thereof, or a splice variant thereof;
• (ii) a nucleic acid coding for a protein having at least 70% identity, at least 80% identity, at least 90% identity, at least 95% identity with SEQ ID NO:
• 4 or a functional fragment thereof;
• (iii) an exogenous nucleic acid capable of hybridizing under stringent conditions with a complementary sequence of any of the nucleic acids according to (i) or (ii);
• or
• (iv) a nucleic acid encoding the same CCoAOMT1 protein as the nucleic acids of (i) to (iii) above, but differing from the nucleic acids of (i) to (iii) above due to the degeneracy of the genetic code;
• operably linked with a promoter and a transcription termination sequence,
• (c) a nucleic acid encoding ABCG37 protein wherein said ABCG37 protein is encoded by
• (i) a nucleic acid having at least 70% identity, at least 80% identity, at least 90% identity, at least 95% identity with SEQ ID NO:
• 5 or a functional fragment thereof, or a splice variant thereof;
• (ii) a nucleic acid coding for a protein having at least 70% identity, at least 80% identity, at least 90% identity, at least 95% identity with SEQ ID NO:
• 6 or a functional fragment thereof;
• (iii) an exogenous nucleic acid capable of hybridizing under stringent conditions with a complementary sequence of any of the nucleic acids according to (i) or (ii);
• or
• (iv) a nucleic acid encoding the same ABCG37 protein as the nucleic acids of (i) to (iii) above, but differing from the nucleic acids of (i) to (iii) above due to the degeneracy of the genetic code;
• operably linked with a promoter and a transcription termination sequence;
• and
• (d) a nucleic acid encoding UGT71C1 protein wherein said UGT71C1 protein is encoded by
• (i) a nucleic acid having at least 70% identity, at least 80% identity, at least 90% identity, at least 95% identity with SEQ ID NO:
• 7 or a functional fragment thereof, or a splice variant thereof;
• (ii) a nucleic acid coding for a protein having at least 70% identity, at least 80% identity, at least 90% identity, at least 95% identity with SEQ ID NO:
• 8 or a functional fragment thereof;
• (iii) an exogenous nucleic acid capable of hybridizing under stringent conditions with a complementary sequence of any of the nucleic acids according to (i) or (ii);
• or
• (iv) a nucleic acid encoding the same UGT71C1 protein as the nucleic acids of (i) to (iii) above, but differing from the nucleic acids of (i) to (iii) above due to the degeneracy of the genetic code;
• operably linked with a promoter and a transcription termination sequence.

A transgenic plant, transgenic plant part, or transgenic plant cell overexpressing an exogenous F6H1 protein optionally in combination with one or more additional exogenous protein(s) selected from the group consisting of a CCoAOMT1 protein, an ABCG37 protein and an UGT71C1 protein,

• (a) wherein said F6H1 protein is encoded by
• (i) a nucleic acid having at least 70% identity, at least 80% identity, at least 90% identity, at least 95% identity with SEQ ID NO:
• 1 or a functional fragment thereof, or a splice variant thereof;
• (ii) a nucleic acid coding for a protein having at least 70% identity, at least 80% identity, at least 90% identity, at least 95% identity with SEQ ID NO:
• 2 or a functional fragment thereof;
• (iii) an exogenous nucleic acid capable of hybridizing under stringent conditions with a complementary sequence of any of the nucleic acids according to (i) or (ii);
• or
• (iv) a nucleic acid encoding the same F6H1 protein as the nucleic acids of (i) to (iii) above, but differing from the nucleic acids of (i) to (iii) above due to the degeneracy of the genetic code;
• operably linked with a promoter and a transcription termination sequence,
• (b) wherein said CCoAOMT1 protein is encoded by
• (i) a nucleic acid having at least 70% identity, at least 80% identity, at least 90% identity, at least 95% identity with SEQ ID NO:
• 3 or a functional fragment thereof, or a splice variant thereof;
• (ii) a nucleic acid coding for a protein having at least 70% identity, at least 80% identity, at least 90% identity, at least 95% identity with SEQ ID NO:
• 4 or a functional fragment thereof;
• (iii) an exogenous nucleic acid capable of hybridizing under stringent conditions with a complementary sequence of any of the nucleic acids according to (i) or (ii);
• or
• (iv) a nucleic acid encoding the same CCoAOMT1 protein as the nucleic acids of (i) to (iii) above, but differing from the nucleic acids of (i) to (iii) above due to the degeneracy of the genetic code;
• operably linked with a promoter and a transcription termination sequence,
• (c) wherein said ABCG37 protein is encoded by
• (i) a nucleic acid having at least 70% identity, at least 80% identity, at least 90% identity, at least 95% identity with SEQ ID NO:
• 5 or a functional fragment thereof, or a splice variant thereof;
• (ii) a nucleic acid coding for a protein having at least 70% identity, at least 80% identity, at least 90% identity, at least 95% identity with SEQ ID NO:
• 6 or a functional fragment thereof;
• (iii) an exogenous nucleic acid capable of hybridizing under stringent conditions with a complementary sequence of any of the nucleic acids according to (i) or (ii);
• or
• (iv) a nucleic acid encoding the same ABCG37 protein as the nucleic acids of (i) to (iii) above, but differing from the nucleic acids of (i) to (iii) above due to the degeneracy of the genetic code;
• operably linked with a promoter and a transcription termination sequence, and
• (d) wherein said UGT71C1 protein is encoded by
• (i) a nucleic acid having at least 70% identity, at least 80% identity, at least 90% identity, at least 95% identity with SEQ ID NO:
• 7 or a functional fragment thereof, or a splice variant thereof;
• (ii) a nucleic acid coding for a protein having at least 70% identity, at least 80% identity, at least 90% identity, at least 95% identity with SEQ ID NO:
• 8 or a functional fragment thereof;
• (iii) an exogenous nucleic acid capable of hybridizing under stringent conditions with a complementary sequence of any of the nucleic acids according to (i) or (ii);
• or
• (iv) a nucleic acid encoding the same UGT71C1 protein as the nucleic acids of (i) to (iii) above, but differing from the nucleic acids of (i) to (iii) above due to the degeneracy of the genetic code;
• operably linked with a promoter and a transcription termination sequence.

A method for the production of a transgenic plant, transgenic plant part, or transgenic plant cell having increased fungal resistance, comprising

• (i) introducing an exogenous nucleic acid encoding the F6H1 protein optionally in combination with one or more exogenous nucleic acid(s) encoding the exogenous protein(s) selected from the group consisting of CCoAOMT1 protein, ABCG37 protein and UGT71C1 protein into a plant, a plant part, or a plant cell,
• (ii) generating a transgenic plant, transgenic plant part, or transgenic plant cell from the plant, plant part or plant cell;
• and
• (iii) expressing the protein(s) encoded by the recombinant vector construct(s),
• wherein the exogenous nucleic acid(s) encoding the F6H9 protein, the CCoAMT1 protein, the ABCG37 protein and/or the UGT71C1 protein are located on the same or different vector constructs,
• (a) wherein said F6H1 protein is encoded by
• (i) a nucleic acid having at least 70% identity, at least 80% identity, at least 90% identity, at least 95% identity with SEQ ID NO:
• 1 or a functional fragment thereof, or a splice variant thereof;
• (ii) a nucleic acid coding for a protein having at least 70% identity, at least 80% identity, at least 90% identity, at least 95% identity with SEQ ID NO:
• 2 or a functional fragment thereof;
• (iii) an exogenous nucleic acid capable of hybridizing under stringent conditions with a complementary sequence of any of the nucleic acids according to (i) or (ii);
• or
• (iv) a nucleic acid encoding the same F6H1 protein as the nucleic acids of (i) to (iii) above, but differing from the nucleic acids of (i) to (iii) above due to the degeneracy of the genetic code
• operably linked with a promoter and a transcription termination sequence,
• (b) wherein said CCoAOMT1 protein is encoded by
• (i) a nucleic acid having at least 70% identity, at least 80% identity, at least 90% identity, at least 95% identity with SEQ ID NO:
• 3 or a functional fragment thereof, or a splice variant thereof;
• (ii) a nucleic acid coding for a protein having at least 70% identity, at least 80% identity, at least 90% identity, at least 95% identity with SEQ ID NO:
• 4 or a functional fragment thereof;
• (iii) a nucleic acid capable of hybridizing under stringent conditions with any of the nucleic acids according to (i) or (ii) or a complementary sequence thereof;
• or
• (iv) a nucleic acid encoding the same CCoAOMT1 protein as the nucleic acids of (i) to (iii) above, but differing from the nucleic acids of (i) to (iii) above due to the degeneracy of the genetic code;
• operably linked with a promoter and a transcription termination sequence,
• (c) wherein said ABCG37 protein is encoded by
• (i) a nucleic acid having at least 70% identity, at least 80% identity, at least 90% identity, at least 95% identity with SEQ ID NO:
• 5 or a functional fragment thereof, or a splice variant thereof;
• (ii) a nucleic acid coding for a protein having at least 70% identity, at least 80% identity, at least 90% identity, at least 95% identity with SEQ ID NO:
• 6 or a functional fragment thereof;
• (iii) an exogenous nucleic acid capable of hybridizing under stringent conditions with a complementary sequence of any of the nucleic acids according to (i) or (ii);
• or
• (iv) a nucleic acid encoding the same ABCG37 protein as the nucleic acids of (i) to (iii) above, but differing from the nucleic acids of (i) to (iii) above due to the degeneracy of the genetic code
• and
• (d) wherein said UGT71C1 protein is encoded by
• (i) a nucleic acid having at least 70% identity, at least 80% identity, at least 90% identity, at least 95% identity with SEQ ID NO:
• 7 or a functional fragment thereof, or a splice variant thereof;
• (ii) a nucleic acid coding for a protein having at least 70% identity, at least 80% identity, at least 90% identity, at least 95% identity with SEQ ID NO:
• 8 or a functional fragment thereof;
• (iii) an exogenous nucleic acid capable of hybridizing under stringent conditions with a complementary sequence of any of the nucleic acids according to (i) or (ii);
• or
• (iv) a nucleic acid encoding the same UGT71C1 protein as the nucleic acids of (i) to (iii) above, but differing from the nucleic acids of (i) to (iii) above due to the degeneracy of the genetic code.