Ligand conjugated quantum dot nanoparticles and methods of detecting dna methylation using same
AssigneeNanoco Technologies Ltd.
InventorImad Naasani
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Abstract
The present disclosure relates to a conjugated quantum dot nanoparticles, to methods of making such conjugated quantum dot nanoparticles, and to methods of detecting DNA methylation using such conjugated quantum dot nanoparticles.
Claims20
A nanoparticle conjugate, wherein a nanoparticle is linked to a DNA methylation specific binding ligand.
A nanoparticle conjugate wherein the nanoparticle is a quantum dot comprising a core semiconductor material, and an outer layer,
- wherein the outer layer comprises a functionalization organic coating linked to a methylation specific binding ligand.
The ligand nanoparticle conjugate of claim 3, wherein the methylation specific binding ligand binds specifically to 5-methylcytosine and/or 5-hydroxymethylcytosine.
The ligand nanoparticle conjugate of claim 4, wherein the methylation specific binding ligand is selected from an anti-5-methylcytosine antibody, an anti-5-hydroxymethylcytosine antibody, an anti-5-formylcytosine antibody, an anti-5-carboxylcytosine antibody, an anti-N6-methyladenine antibody and any combination thereof.
A process for preparing a ligand nanoparticle conjugate comprising coupling a nanoparticle with a DNA methylation specific binding ligand, wherein the nanoparticle comprises a quantum dot comprising a core semiconductor material, and an outer layer, wherein the outer layer comprises a carboxyl group.
Dependencies are inferred from the claim text.