Back to results
US 2018/0008984 A1Published application
Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
Assignee10x Genomics, Inc.
InventorsRajiv Bharadwaj, Kevin Ness, Debkishore Mitra, Donald Masquelier, Anthony Makarewicz, Christopher Hindson, Benjamin Hindson, Serge Saxonov
Life of this patent
Estimating the term…
Abstract
The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyses and characterization.
Claims30
A microfluidic system, comprising:
- a microfluidic channel network comprising at least first, second and third channels in fluid communication with a droplet generation junction;
- a first fluid source fluidly connected to the first channel and comprising a first fluid that comprises an aqueous fluid and a plurality of microcapsules;
- a second fluid source fluidly connected to the second channel and comprising a second fluid that is immiscible with the aqueous fluid;
- and
- a flow control system connected to the microfluidic channel network that (i) subjects the first fluid from the first fluid source to flow along the first channel in a manner such that microcapsules from the plurality of microcapsules flow at a flow frequency that is substantially regular in the first channel as determined by a number of microcapsules that flow past a given point in the first channel within a one second period of time, and (ii) subjects the second fluid from the second fluid source to flow along the second channel, such that the first fluid and the second fluid meet at the droplet generation junction to generate a plurality of droplets comprising microcapsules from the plurality of microcapsules, which plurality of droplets flow along the third channel.
2 more elements
A microfluidic system, comprising:
- a microfluidic channel network comprising at least first, second and third channels in fluid communication with a droplet generation junction;
- a first fluid source fluidly connected to the first channel and comprising a first fluid that comprises an aqueous fluid and a plurality of microcapsules;
- a second fluid source fluidly connected to the second channel and comprising a second fluid that is immiscible with the aqueous fluid;
- and
- a flow control system connected to the microfluidic channel network that (i) subjects the first fluid from the first fluid source to flow along the first channel in a manner such that microcapsules from the plurality of microcapsules flow at a flow frequency greater than 50 Hz at a coefficient of variation of less than 30% in the first channel as determined by a number of microcapsules that flow past a given point in the first channel within a one second period of time, and (ii) subjects the second fluid from the second fluid source to flow along the second channel, such that the first fluid and the second fluid meet at the droplet generation junction to generate a plurality of droplets comprising microcapsules from the plurality of microcapsules, which plurality of droplets flow along the third channel.
2 more elements
Dependencies are inferred from the claim text.