Dually Derivatized Chitosan Nanoparticles and Methods of Making and Using the Same for Gene Transfer In Vivo

A method of treating diabetes comprising administering a therapeutically effective amount of a therapeutic nucleic acid encoding insulin, a glucagon antagonist, GLP-1 or leptin to a target tissue in a patient, wherein said administering comprises contacting said target tissue with a dually derivatized (DD) chitosan nucleic acid polyplex, said DD chitosan nucleic acid polyplex comprising a chitosan-derivative nanoparticle comprising chitosan coupled with gluconic acid and arginine, and said therapeutic nucleic acid.

A method of treating inflammatory bowel disease comprising administering a therapeutically effective amount of a therapeutic nucleic acid encoding IL-10, a TNFα antagonist, or an IL-17 antagonist to a target tissue in a patient, wherein said administering comprises contacting said target tissue with a dually derivatized (DD) chitosan nucleic acid polyplex, said DD chitosan nucleic acid polyplex comprising a chitosan-derivative nanoparticle comprising chitosan coupled with gluconic acid and arginine, and said therapeutic nucleic acid.

A method of treating of obesity comprising administering a therapeutically effective amount of a therapeutic nucleic acid encoding leptin, cholecystokinin, PYY or GLP-1 to a target tissue in a patient, wherein said administering comprises contacting said target tissue with a dually derivatized (DD) chitosan nucleic acid polyplex, said DD chitosan nucleic acid polyplex comprising a chitosan-derivative nanoparticle comprising chitosan coupled with gluconic acid and arginine, and said therapeutic nucleic acid.