Preparation Method of the Albumin Peptide Combination and the Action of Inhibiting the Proliferation of Cancer Cells Thereof

A preparation method of an albumin peptide combination having the action of inhibiting the proliferation of cancer cells, wherein the albumin peptide combination, having an action of inhibiting a proliferation of cancer cells, comprising:

• raw materials including an albumin enzymatic hydrolysate and a coix seed extract under mass ratio of 5:2 to 5, wherein a content of a solid matter of the albumin enzymatic hydrolysate is 15% to 35% and a content of a solid matter of the coix seed extract is 10% to 30%, wherein the preparation method comprises the steps of:
• (1) adding albumin into water that is 5 to 20 times to a mass thereof, heating the albumin and water mixture to 40° C. to 50° C., and homogenizing for producing a solution;
• (2) adjusting pH of the solution after the homogenizing of step (1) to 7 to 9, adding an alkaline protease at 3% to 5% of the mass of the solution to perform enzymatic hydrolysis reaction for 5 h to 10 h for producing a hydrolysis solution;
• (3) heating the hydrolysis solution of the step (2) to boil, deactivating for 0.5 h to 2 h;
• (4) cooling the hydrolysis solution after the deactivating of the step (3) to 60° C. to 80° C., and performing filtration to obtain the albumin enzymatic hydrolysate;
• (5) decocting first coix seeds with boiling water for 3 h to 5 h, wherein an amount of water is 10 to 15 times of a mass of the first coix seeds, subsequently, performing filtration;
• (6) decocting second coix seeds with boiling water for 1 h to 3 h, wherein an amount of water is 5 to 10 times of a mass of the second coix seed, subsequently, performing filtration;
• (7) combining filtrates of the two filtrations of the step (5) and the step (6) to obtain the coix seed extract;
• (8) performing solid matter determination for the filtrate of the albumin peptide enzymatic hydrolysate and the coix seed extract respectively, mixing the albumin peptide enzymatic hydrolysate and the coix seed extract under appropriate ratio;
• and
• (9) performing low temperature vacuum concentration to the mixed liquid of the step (8), wherein temperature is 40° C. to 60° C. and pressure is 0.03 MPa to 0.07 MPa, subsequently, spray drying, wherein the temperature of the wind at inlet is 180° C. to 220° C. and the temperature of the wind at outlet is 80° C. to 110° C., preparing the albumin peptide combination having the action of inhibiting the proliferation of cancer cells.