Cell penetrating antibodies
AssigneeCity of Hope
InventorsAndreas Herrmann, Hua Yu
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Abstract
Provided herein are cell penetrating conjugates. The conjugates include a non-cell penetrating protein attached to a phosphorothioate nucleic acid or phosphorothioate polymer backbone through a non-covalent linker including abiotin-binding domain and a biotin domain, wherein the phosphorothioate nucleic acid or phosphorothioate polymer backbone enhances intracellular delivery of the non-cell penetrating protein. Also provided are compositions and kits comprising the conjugates.
Claims67
A cell-penetrating conjugate comprising:
- (i) a non-cell penetrating protein;
- (ii) a phosphorothioate nucleic acid;
- and
- (iii) a non-covalent linker attaching said phosphorothioate nucleic acid to said non-cell penetrating protein, said non-covalent linker comprising a biotin-binding domain non-covalently attached to a biotin domain;
- and
- wherein said phosphorothioate nucleic acid enhances intracellular delivery of said non-cell penetrating protein.
A method of forming a cell penetrating conjugate, said method comprising contacting a non-cell penetrating protein with a phosphorothioate nucleic acid, wherein said non-cell penetrating protein is attached to a first member of a biotin binding pair and said phosphorothioate nucleic acid is attached to a second member of said biotin binding pair, thereby forming a cell penetrating conjugate comprising a non-covalent bond between a biotin domain and biotin-binding domain.
Dependencies are inferred from the claim text.