Adherent stromal cells derived from placentas of multiple donors and uses thereof
AssigneePluristem Ltd.
InventorsZami Aberman, Ora Burger, Shai Meretzki
Life of this patent
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Abstract
Pharmaceutical compositions comprising adherent stromal cells (ASCs) are provided. The ASCs are obtained from at least two donors. Articles of manufacture comprising the pharmaceutical compositions together with a delivery device for administering the ASCs to a subject are also provided. Also provided are methods of treating various diseases and conditions that are treatable by administering ASCs to a subject in need of treatment.
Claims24
A method of treating at least one condition that can be treated by administration of placental-derived adherent stromal cells (ASCs) to a subject in need thereof, the method comprising administering to the subject an effective amount of adherent stromal cells (ASCs), wherein the ASCs are negative for the marker CD200, wherein the administered ASCs comprise ASCs from at least two donor placentas, wherein the at least two donors have at least two different HLA genotypes, and wherein the at least one condition is selected from stem cell deficiency, heart disease. a neurodegenerative disorder, cancer, stroke, burns, loss of tissue, loss of blood, anemia, an autoimmune disease, ischemia, skeletal muscle regeneration, neuropathic pain, a compromised hematopoietic system, geriatric diseases, and a medical condition requiring connective tissue regeneration and/or repair.
A method of cell expansion, comprising:
- (i) inoculating a cell culture system comprising a culture medium with adherent stromal cells (ASC) from human placenta or adipose tissue, wherein said cell culture system comprises three-dimensional (3D) carriers comprising a fabric matrix;
- (ii) culturing the ASC under conditions that support expansion of the cells;
- and
- (iii) detaching the expanded ASC from the matrix in a viable form.
A method of preparing a pharmaceutical composition for transplantation, comprising:
- (i) inoculating a cell culture system comprising a culture medium with adherent stromal cells (ASC) from human placenta or adipose tissue, wherein said cell culture system comprises three-dimensional (3D) carriers comprising a fabric matrix;
- (ii) culturing the ASC under conditions that support expansion of the cells;
- (iii) detaching the expanded ASC from the matrix in a viable form;
- and
- (iv) suspending the expanded ASC in a pharmaceutically acceptable carrier.
Dependencies are inferred from the claim text.