Wound healing
AssigneeCytosolv, Inc.
InventorsChristopher Thanos, Briannan Bintz
Life of this patent
Estimating the term…
Abstract
The invention relates to methods and compositions for improving wound healing and in particular for preventing scar formation and thus loss of function that can occur in injured tissues during the natural wound healing process. Particularly, although by no means exclusively, the invention relates to the healing of chronic wounds such as diabetic ulcers.
Claims50
A therapeutic composition comprising isolated or purified choroid plexus (CP) secreted proteins, wherein the CP secreted proteins are isolated or purified from CP conditioned media.
A therapeutic wound healing composition comprising encapsulated CP cells.
A cosmetic composition comprising encapsulated CP cells wherein the composition is formulated for topical administration.
A method of improving tissue wound healing comprising the steps of administering an effective amount of a therapeutic composition comprising isolated or purified CP secreted proteins to a human or non-human patient in need thereof, wherein the CP secreted proteins are isolated or purified from CP conditioned media.
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A pharmaceutical composition comprising isolated or purified CP secreted proteins wherein the CP secreted proteins are isolated or purified from CP conditioned media, together with a pharmaceutically acceptable carrier.
A pharmaceutical composition comprising encapsulated CP cells together with a pharmaceutically acceptable carrier.
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A method of manufacturing a therapeutic composition comprising isolated or purified CP secreted proteins, said method comprising the steps:
- a) culturing CP cells in vitro in basal growth media for a time sufficient for the CP cells to secrete proteins into the media;
- b) extracting the CP conditioned media;
- c) lyophilizing the CP conditioned media;
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- d) optionally dialyzing the CP conditioned media either before or after step c).
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A method of manipulating the profile of CP secreted proteins from CP cells in vitro comprising the steps:
- a) culturing CP cells in a basal growth medium in vitro;
- b) adding cellular matrix proteins to the culture medium to initiate the formation of CP capsuloids;
- c) culturing the capsuloids for a time sufficient to increase the number of secretory vesicles in the capsuloids;
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- d) producing CP conditioned media containing an altered CP secreted protein profile compared to CP cells cultured in basal growth media alone.
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A method of treating a wound in a human or non-human patient comprising the steps of administering an effective amount of a therapeutic composition comprising isolated or purified CP secreted proteins to a human or non-human patient in need thereof, wherein the CP secreted proteins are isolated or purified from CP conditioned media.
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A therapeutic composition comprising two or more isolated or purified choroid plexus (CP) secreted proteins, wherein the CP secreted proteins are selected from Table 5 or Table 6, and a pharmaceutically acceptable excipient.
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A method for improving hair growth in a mammal identified as in need thereof comprising the steps of administering an effective amount of a therapeutic composition comprising isolated or purified CP secreted proteins to the mammal, wherein the CP secreted proteins are isolated or purified from CP conditioned media.
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Dependencies are inferred from the claim text.