Purified mesenchymal stem cell compositions and methods of purifying mesenchymal stem cell compositions
AssigneeMesoblast International Sarl
InventorsAlla Danilkovich, Robert E. Newman, JR., Samson Tom, Christopher Ton, Zhanling Wang, Randell G. Young
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Abstract
One or more purified mesenchymal stem cell pharmaceutical compositions and methods of manufacture utilizing centrifugal filtration are disclosed. Threshold limits for intravenous administration of mesenchymal stem cell pharmaceutical compositions comprising residual animal products are also disclosed.
Claims26
A method of preparing a pharmaceutically acceptable composition comprising a plurality of mesenchymal stem cells and mesenchymal stem cell aggregates comprising the steps of:
- (i) obtaining a mesenchymal stem cell suspension comprising a plurality of mesenchymal stem cells and mesenchymal stem cell aggregates;
- (ii) contacting the suspension with a wash solution to create a mixed suspension;
- (iii) agitating the mixed suspension with a centrifugal filtration device until the D90 of the mesenchymal stem cell aggregates in the mesenchymal stem cell suspension is less than about 150 μm;
- (iv) recovering the pharmaceutically acceptable composition.
11-24. (canceled)
A method of preventing pulmonary embolism in a subject, wherein pulmonary embolism is caused by aggregation of mesenchymal stem cells, comprising administering a pharmaceutically acceptable composition comprising a plurality of mesenchymal stem cells and mesenchymal stem cell aggregates, wherein the aggregates comprise a D90 of less than about 150 μm.
Dependencies are inferred from the claim text.