Method for producing benzazoloquinolium (bqs) salts and using the biological activity of the composition
AssigneeSistema Universitario Ana G. Mendez, Inc.
InventorsOsvaldo Cox, Beatriz Zayas
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Abstract
Disclosed is the synthesis procedure for benzazolo[3,2-a]quinolinium chloride salts and the inclusion of chloro-substituent, amino-substituent, and nitro-substituent resulting in several compounds. The compounds are further used as markers due to their fluorescent properties including in hypoxic environments. This disclosure further describes anti-cancer screening of two BQS, namely, 7-benzyl-3-aminobenzimidazo[3,2-a]quinolinium chloride (ABQ-48: NSC D-763307) and the corresponding 7-benzyl-3-nitrobenzimidazo[3,2-a]quinolinium chloride (NBQ 48: NSC D-763303).
Claims21
A method of treating cancer cells selected from the group consisting of non-small cell lung cancer, central nervous system cancer, melanoma, ovarian cancer, renal cancer, prostate cancer, and breast cancer in a mammal comprising administering to said mammal, a therapeutically effective amount of a benzazolo[3,2-a]quinolinium chloride salt compound of formula
- wherein R1 is selected from a group consisting of 3-NH2 and 3-NO2.
A method of detecting cells under hypoxia comprising administering a nitro-substituted benzazolo[3,2-a]quinolinium chloride salt compound of formula
- wherein R1 is 3-NO2 comprising:
- said nitro-substituted benzazolo[3,2-a]quinolinium chloride salt compound and at least one cell;
- wherein said at least one cell is exposed to the nitro-substituted benzazolo[3,2-a] quinolinium chloride salt compound to mark said cell;
- and
- wherein the fluorescence of a sample of said cell is measured after said exposure.
A method of measuring metabolic activity of cells comprising:
- administering a nitro-substituted benzazolo[3,2-a]quinolinium chloride salt compound of formula
- wherein R1 is 3-NO2 to measure metabolic activity in cells under hypoxia comprising:
- said nitro-substituted benzazolo[3,2-a]quinolinium chloride salt and at least one cell;
- wherein said cell is exposed to the nitro-substituted benzazolo[3,2-a] quinolinium chloride salt compound;
- and
- wherein the fluorescence of a sample of said cell is measured after said exposure indicative of a metabolically active hypoxic cell.
Dependencies are inferred from the claim text.