Active substance for preventing degeneration of hearing, composition comprising same, and preparation method thereof
AssigneeGrape King Bio Ltd.
InventorsYin-Ching Chan, Chin-Chu Chen, Li-Ya Lee, Wan-Ping Chen
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Abstract
The present invention discloses the active substances for preventing hearing deterioration, its preparation method, the pharmaceutical composition containing the active substances, and the preparation method of the pharmaceutical composition. The preparation method of the active substances is performed by plate cultivation, flask cultivation and fermentation tank cultivation, to obtain the active substances of Hericium erinaceus mycelia in powder form. The powder of H. erinaceus mycelia is proved to have the effect of preventing hearing deterioration.
Claims15
-13. (canceled)
A pharmaceutical composition for preventing a hearing deterioration, comprising an active substance of a Hericium erinaceus, and one selected from the group consisting of a biologically acceptable carrier, an excipient, a diluent and an adjuvant, wherein the active substance is prepared by a method comprising:
- (a) inoculating an H. erinaceus mycelium on an agar plate to be incubated;
- (b) inoculating the incubated mycelium in step (a) into a first medium on a small scale to be incubated;
- and
- (c) inoculating the incubated mycelium in step (b) into a second medium on a large scale to be incubated to obtain a fermented medium containing the active substance.
A method for preventing a hearing deterioration with an active substance of a Hericium erinaceus prepared by a method comprising:
- (a) inoculating an H. erinaceus mycelium on an agar plate to be incubated;
- (b) inoculating the incubated mycelium in step (a) into a first medium on a small scale to be incubated;
- and
- (c) inoculating the incubated mycelium in step (b) into a second medium on a large scale to be incubated to obtain a fermented medium containing the active substance.
A treating method for preventing a hearing deterioration with an active substance of a Hericium erinaceus prepared by steps comprising:
- (a) inoculating an H. erinaceus mycelium on an agar plate to be incubated;
- (b) inoculating the incubated mycelium in step (a) into a first medium on a small scale to be incubated;
- and
- (c) inoculating the incubated mycelium in step (b) into a second medium on a large scale to be incubated to obtain a fermented medium containing the active substance.
Dependencies are inferred from the claim text.