Back to results
US 2018/0004894 A1Published application
Methods for Sequence-Directed Molecular Breeding
AssigneeMonsanto Technology LLC
InventorsStanton B. DOTSON, Fenggao DONG, Frederic ARCHARD, Sam EATHINGTON, Nengbing TAO, Zoe McKINESS f/n/a McCUDDIN
Life of this patent
Estimating the term…
Abstract
The present invention provides breeding methods and compositions to enhance the germplasm of a plant by the use of direct nucleic acid sequence information. The methods describe the identification and accumulation of preferred nucleic acid sequences in the germplasm of a breeding population of plants.
Claims11
1-4. (canceled)
A method of identifying a phenotypic trait of interest in a plant, the method comprising:
- providing a plurality of plant DNA samples, wherein at least one of the plant DNA samples comprises a polymorphism associated with a phenotypic trait of interest;
- amplifying one or more selected loci in the genome of two or more of the plant DNA samples, wherein each of said selected loci is amplified with at least one specific primer and tagged with at least one DNA tag;
- combining at least two of the amplified DNA samples to create a pool of amplified DNA samples;
- sequencing the pool of amplified DNA samples;
- comparing the sequence information from the amplified DNA samples to a consensus sequence to identify the presence or absence of the polymorphism, wherein the individual DNA samples are identified by the DNA tags;
- and
- selecting one or more plants based on the presence or absence of the polymorphism in the plant DNA sample.
4 more elements
A method of identifying a phenotypic trait of interest in a plant, the method comprising:
- providing a plurality of plant DNA samples, wherein at least one of the plant DNA samples comprises a polymorphism associated with a phenotypic trait of interest;
- amplifying one or more selected loci in the genome of two or more of the plant DNA samples, wherein each of said selected loci is amplified with at least one specific primer comprising at least one DNA tag and at least one allele-specific tag;
- combining at least two of the amplified DNA samples to create a pool of amplified DNA samples;
- sequencing the allele-specific tags in the pool of amplified DNA samples;
- comparing the sequence information from the allele-specific tags to a known library of allele-specific tags to identify the presence or absence of the polymorphism in individual DNA samples, wherein the individual DNA samples are identified by the DNA tags;
- and
- selecting one or more plants based on the presence or absence of the polymorphism in the plant DNA samples.
4 more elements
Dependencies are inferred from the claim text.