Method for producing antibody reagent
AssigneeSysmex Corporation
InventorsSeiichi HASHIDA, Aya MORIMOTO, Toshihiro WATANABE, Takahiro YAMAGAITO
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Abstract
Disclosed is a method for producing an antibody reagent for detecting a test substance in a sample by an immune complex transfer method. The method comprises the steps of: bringing an antibody solution comprising a labeled antibody capable of binding to the test substance into contact with a solid phase used in the immune complex transfer method; and separating the solid phase and the antibody solution to prepare the antibody reagent from the antibody solution.
Claims20
A method for producing an antibody reagent for detecting a test substance in a sample by an immune complex transfer method, comprising the steps of:
- bringing an antibody solution comprising a labeled antibody capable of binding to the test substance into contact with a solid phase used in the immune complex transfer method and
- separating the solid phase and the antibody solution to prepare the antibody reagent from the antibody solution, wherein:
- the labeled antibody comprises a label,
- a binding substance is immobilized on the solid phase,
- in the immune complex transfer method, a capture antibody that specifically binds to the test substance is used, and the capture antibody comprises a binding partner capable of specifically binding to the binding substance.
A method for increasing specificity of an antibody reagent which is used for detecting a test substance in a sample by an immune complex transfer method, comprising the steps of:
- bringing an antibody solution comprising labeled antibodies capable of binding to the test substance into contact with a solid phase used in the immune complex transfer method, whereby preparing a mixture comprising:
- the labeled antibody which nonspecifically binds to the solid phase;
- and the labeled antibody which does not bind to the solid phase, and
- removing the solid phase from the mixture and retrieving the solution as the antibody reagent, wherein:
- the labeled antibody comprises a label,
- a binding substance is immobilized on the solid phase,
- in the immune complex transfer method, a capture antibody that specifically binds to the test substance is used, and the capture antibody comprises a binding partner capable of specifically binding to the binding substance.
A method for purifying an antibody reagent which is used for detecting a test substance in a sample by an immune complex transfer method, comprising the steps of:
- providing an antibody solution comprising labeled antibodies prepared by complexing a label and an antibody, the labeled antibodies being capable of binding to the test substance
- bringing the antibody solution into contact with a solid phase used in the immune complex transfer method, whereby preparing a mixture comprising:
- the labeled antibody which nonspecifically binds to the solid phase;
- and the labeled antibody which does not bind to the solid phase, and
- removing the solid phase from the mixture and retrieving the solution as the antibody reagent, wherein:
- the labeled antibody comprises a label,
- a binding substance is immobilized on the solid phase,
- in the immune complex transfer method, a capture antibody that specifically binds to the test substance is used, and the capture antibody comprises a binding partner capable of specifically binding to the binding substance.
Dependencies are inferred from the claim text.