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US 2018/0003705 A1Published application
Biosensor for use with a surface plasmon resonance (spr) sensor
OwnerRonald Koder, Peter Schnatz +4inventors
InventorsRonald Koder, Peter Schnatz, Joseph Brisendine, David Crouse, Lori Lepak, Igor Bendoym
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Abstract
A surface plasmon resonance (SPR) sensor is provided that has enhanced sensitivity. The sensor's plasmonic chip has intrinsically disordered proteins (IDPs) that undergo enzyme-free folding upon binding to an analyte. This binding results in a detectable change in refractive index and thereby permits detection of the analyte.
Claims18
An optical surface plasmon resonance (SPR) biosensor comprising
- a light source;
- a plasmonic chip with a plurality of intrinsically disordered proteins (IDPs) comprising negatively charged residues, the plurality of IDPs being covalently bond to a plasmonic chip and providing a binding site for binding to a predetermined analyte, wherein the IDPs undergo enzyme-free folding from an extended state to a folded state upon binding to the predetermined analyte;
- an optical detector for detecting light from the light source after the light has interacted with the plasmonic chip, the plasmonic chip being disposed between the light source and the optical detector such that the optical detector detects transmitted light.
An optical surface plasmon resonance (SPR) biosensor comprising
- a light source
- a plasmonic chip with a plurality of intrinsically disordered proteins (IDPs) comprising negatively charged residues, the plurality of IDPs being covalently bond to a plasmonic chip and providing a binding site for binding to a predetermined analyte, wherein the IDPs have between two-hundred and three-hundred residues and undergo enzyme-free folding from an extended state to a folded state upon binding to the predetermined analyte, wherein at least 15% of the residues are negatively charged and fewer than 5% of the residues are positively charged;
- an optical detector for detecting light from the light source after the light has interacted with the plasmonic chip.
A method for detecting an analyte, the method comprising steps of:
- exposing a sample to a biosensor configured for use with a surface plasmon resonance (SPR) sensor, the biosensor comprising a plurality of intrinsically disordered proteins (IDPs) comprising negatively charged residues, the plurality of IDPs being covalently bond to a plasmonic chip and providing a binding site for binding to an analyte, wherein the IDPs undergo enzyme-free folding from an extended state to a folded state upon binding to the analyte;
- wherein the sample comprises an analyte;
- permitting the analyte to bind to at least one IDP in the plurality of IDPs and induce a conformational change in the at least one IDP from a first confirmation to a second confirmation, wherein the plasmonic chip has a first refractive index when the at least one IDP is in the first confirmation and a second refractive index when the at least one IDP is in the second confirmation;
- illuminating the plasmonic chip with light;
- detecting the second refractive index, thereby detecting the analyte.
2 more elements
Dependencies are inferred from the claim text.