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AssigneeSenomyx, Inc.
InventorsXiaodong LI, Lena LUUKKONEN, Hong XU
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The invention relates to compounds that specifically bind a T1R1/T1R3 or T1R2/T1R3 receptor or fragments or sub-units thereof. The present invention also relates to the use of hetero-oligomeric and chimeric taste receptors comprising T1R1/T1R3 and T1R2/T1R3 in assays to identify compounds that respectively respond to umami taste stimuli and sweet taste stimuli. Further, the invention relates to the constitutive of cell lines that stably or transiently co-express a combination of T1R1 and T1R3; or T1R2 and T1R3; under constitutive or inducible conditions. The use of these cells lines in cell-based assays to identify umami and sweet taste modulatory compounds is also provided, particularly high throughput screening assays that detect receptor activity by use of fluorometric imaging.
A method of identifying one or more compounds that putatively modulate or enhance sweet taste perception comprising (i) screening one or more compounds in a first binding assay that identifies whether said one or more compounds specifically bind to a T1R2/T1R3 receptor composed of hTIR2/hTIR3 and (ii) conducting a second binding assay using compounds identified in the first binding assay to determine whether said identified compounds bind to rTIR2/rTIR3 and (iii) selecting compounds that putatively modulate or enhance sweet taste perception which are identified using the binding assays of (i) and which do not bind in the binding assay of (ii).
9-10. (canceled)
18-62. (canceled)
Dependencies are inferred from the claim text.