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US 2018/0003700 A1Published application
Method for the formation and use of an immunolabeling complex
AssigneeKromnigon AB
InventorsPer Fogelstrand, Ulf Yrlid
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Abstract
The present invention generally relates to method for forming an immunolabeling complex, the immunolabeling complex comprising a labeled monovalent biotin-binding composition. The invention also related to the use of the antibody-reporter molecule complex for detecting a target in a sample.
Claims22
A method for forming an immunolabeling complex, wherein the method comprises:
- selecting a first biotinylated primary antibody;
- selecting a first monovalent biotin-binding composition labeled with a first reporter element, and
- mixing, in a reaction vessel, the first biotinylated primary antibody with the first labeled monovalent biotin-binding composition.
A method for detecting a target molecule in a sample, wherein the method comprises:
- contacting a first immunolabeling complex formed according to any one of the preceding claims with the sample;
- incubating the sample for a time sufficient to permit the first immunolabeling complex to selectively bind to the target;
- and
- detecting the target.
1 more element
Dependencies are inferred from the claim text.