Nucleated red blood cell warning method and device, and flow cytometer using the same

A nucleated red blood cell warning device for warning whether nucleated red blood cells exist in a blood sample, comprising a processor, wherein said processor is configured to:

• acquire forward-scattered light information, side-scattered light information and fluorescence information when cells in said blood sample pass through a detection region of a flow cytometer;
• generate a side-scattered light-fluorescence dot plot according to said side-scattered light information and said fluorescence information and classify leucocytes into at least four groups:
• lymphocyte population, monocyte population, eosinophil population, and neutrophil and basophil population;
• generate a forward-scattered light-fluorescence dot plot of said blood sample according to said forward-scattered light information and said fluorescence information, wherein said forward-scattered light-fluorescence dot plot comprises a leucocyte population region;
• and
• acquire a predetermined feature region of said forward-scattered light-fluorescence dot plot, perform statistics on an amount of characterization cells in said predetermined feature region, determine whether said amount of said characterization cells exceeds a predetermined threshold value, and provide a warning when it is determined that said amount of said characterization cells exceeds said predetermined threshold value, wherein said predetermined feature region is located at a left side of said leucocyte population region.

A flow cytometer, comprising:

• a sampling device that draws a blood sample, said blood sample containing at least leucocytes;
• a preprocessing device that preprocesses said blood sample to obtain a processed blood sample, said preprocessing comprising performing fluorescence labeling on cells in said blood sample;
• a detection device that causes said cells in said processed blood sample to pass through a detection region one by one, and detects forward-scattered light information, side-scattered light information and fluorescence information of said cells in said processed blood sample;
• and
• an analysis device that generates a forward-scattered light-fluorescence dot plot of said blood sample according to said forward-scattered light information and said fluorescence information detected by said detection device, acquires a predetermined feature region of said forward-scattered light-fluorescence dot plot, performs statistics on an amount of characterization cells in said predetermined feature region, determines whether said amount of said characterization cells exceeds a predetermined threshold value, and provides a warning when it is determined that said amount of said characterization cells exceeds said predetermined threshold value, wherein said forward-scattered light-fluorescence dot plot comprises a leucocyte population region and said predetermined feature region is located at a left side of said leucocyte population region.

A nucleated red blood cell warning method for warning whether nucleated red blood cells exist in a blood sample, comprising:

• providing a flow cytometer and a blood sample containing leucocytes;
• preprocessing said blood sample so as to obtain a processed blood sample, said preprocessing comprising performing fluorescent labeling on cells in said blood sample;
• injecting said processed blood sample into said flow cytometer, and collecting forward-scattered light information, side-scattered light information and fluorescence information of said cells;
• classifying, according to said side-scattered light information and said fluorescence information, said leucocytes into at least four groups:
• lymphocyte population, monocyte population, eosinophil population, and neutrophil and basophil population;
• generating, according to said forward-scattered light information and said fluorescence information, a forward-scattered light-fluorescence dot plot of said blood sample, said forward-scattered light-fluorescence dot plot comprising a leucocyte population region;
• and
• acquiring a predetermined feature region of said forward-scattered light-fluorescence dot plot, performing statistics on an amount of characterization cells in said predetermined feature region, determining whether said amount of said characterization cells exceeds a predetermined threshold value, and providing a warning when it is determined that said amount of said characterization cells exceeds said predetermined threshold value, wherein said predetermined feature region is located at a left side of said leucocyte population region.