Efficient arrays of amplified polynucleotides

A method of determining sequence information for a plurality of different target polynucleotides, said method comprising:

• providing a plurality of macromolecular structures, each containing one of the plurality of target polynucleotides and an adaptor oligonucleotide;
• providing a surface that comprises a plurality of discrete spaced apart regions to which the macromolecular structures will bind, wherein the discrete spaced apart regions are surrounded by inert inter-regional areas;
• arraying the macromolecular structures amongst the discrete regions on the surface such that a majority of the discrete regions is each occupied by a single macromolecular structure;
• amplifying the target polynucleotides in the macromolecular structures arrayed on the surface by bridge PCR (polymerase chain reaction);
• and
• obtaining sequence reads of the target polynucleotides from products of the bridge PCR.