Methods for increasing mannose content of recombinant proteins

A method for modulating mannose 5 on a recombinant protein during a mammalian cell culture process comprising limiting the amount of glucose in the cell culture medium, wherein the concentration of the glucose is from about 0 to 6 g/L, and supplementing the cell culture medium with galactose or sucrose, wherein the concentration of galactose is from 6-13 g/L or the concentration of sucrose is from about 16-24 g/L.