Compositions and methods thereof for down-regulation of genes following oral delivery of an rnai molecule bioencapsulated within plant cells
AssigneeThe Trustees of the University of Pennsylvania
InventorHenry Daniell
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Abstract
Compositions and methods for down-regulating genes through oral administration of RNAi molecule encapsulated in plant cells are provided.
Claims26
A recombinant plant plastid or viral expression vector which comprises, as operably linked components in the 5′ to 3′ direction of translation, a promoter operable in said plant, a heterologous polynucleotide sequence coding for at least one RNAi molecule selected from the group comprising dsRNA, siRNA, shRNA, and miRNA and a transcription terminator functional in said plant, said vector optionally encoding a nucleic acid encoding a selectable marker.
A method of treating cancer or an immune disorder, comprising oral administration to a subject an edible plant or plant portion comprising a therapeutically effective amount of an RNAi molecule selected from the group comprising dsRNA, siRNA, shRNA, and miRNA, expressed from a chloroplasts, said RNAi molecules targeting a gene which aberrantly causes or regulates said cancer or immune disorder.
Dependencies are inferred from the claim text.