Brassica napus seed specific promoters identified by microarray analysis
AssigneeDow AgroSciences LLC
InventorsHolly Jean BUTLER, Scott Alan BEVAN, Cory M. Larsen, William MOSKAL, Delkin Orlando GONZALEZ
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Abstract
Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements obtained from Brassica napus.
Claims33
A gene expression cassette comprising a promoter operably linked to a transgene, wherein the promoter comprises a polynucleotide that hybridizes under stringent conditions to a polynucleotide probe comprising a sequence identity of at least 90% to a complement of SEQ ID NO:1.
The gene expression cassette of claim 3, wherein the polynucleotide comprises an intron having at least 90% sequence identity to an intron selected from the group consisting of a rice actin intron, a maize ubiquitin intron, and an Arabadiopsis thaliana ubiquitin 10 intron.
A transgenic cell comprising a synthetic polynucleotide that hybridizes under stringent conditions to a polynucleotide probe comprising a sequence identity of at least 90% to a complement of SEQ ID NO:1.
A purified polynucleotide sequence, wherein the purified polynucleotide sequence comprises a polynucleotide that hybridizes under stringent conditions to a polynucleotide probe comprising a sequence identity of at least 90% to a complement of SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.