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AssigneeKuehnle AgroSystems, Inc.
InventorsRobert J. Schurr, Adelheid R. Kuehnle
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The invention pertains to a method for synthesizing a product of interest by culturing a microalgal cell producing the product of interest in the dark in a culture medium comprising an organic acid as a fixed carbon source, wherein the microalgal cell is a facultative heterotroph. The product of interest can be a microalgal biomass, a pigment, terpene, recombinant molecule, biogas, or a precursor thereof. In an embodiment, the culture medium comprises urea as a primary source of nitrogen. In one embodiment, the microalgal cell belongs to the order Chlamydomonadales. A method of identifying and isolating a microalgal cell having a preferred characteristic that is suitable for synthesis of a product of interest is also provided, the method comprising identifying and isolating a non-mutagenized or recombinant microalgal cell from a microalgal culture using a fluorescence activated cell sorting technique and/or a phototaxic response.
A method for synthesizing a product of interest, the method comprising:
A facultative heterotrophic microalgal cell from the order Chlamydomonadales, wherein said cell produces a product when grown in the dark in a medium containing only organic acid as a primary source of carbon and before cell differentiation occurs;
A facultative heterotrophic microalgal cell from the order Chlamydomonadales, wherein said cell produces a product when grown in the dark in a medium containing only organic acid as a primary source of carbon and before cell differentiation occurs;
A method of identifying and isolating a microalgal cell that is suitable for synthesis of a product of interest, the method comprising:
A colorant, feed additive, human nutrient supplement, or personal care or cosmetics ingredient obtained from a heterotrophic microalgal biomass of Chlamydomonadales having a composition comprising more than 0.7% dry weight carotenoids, more than 40% dry weight protein, less than 10% dry weight ash, and a mixture of two or more carotenoids.
Dependencies are inferred from the claim text.