Synthetic carbon fixation pathways
AssigneeInvista North America S.A.R.L.
InventorsAchuthanunni Chokkathukalam, Alex Van Eck Conradie, Ramdane Haddouche, Satnam Surae, Katherine Louise Tibbles
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Abstract
The present disclosure relates to methods for more efficiently recycling reduced electron carriers in a hydrogen-oxidizing microorganism with an operable Calvin-Benson cycle; synthetic carbon fixation pathways that recycle reduced electron carriers more efficiently than the Calvin-Benson cycle, such as methods for enzymatically converting carbon dioxide to formate and assimilating the resulting formate into central carbon metabolism; methods for producing biochemical products; and recombinant hosts utilizing one or more synthetic carbon fixation pathways.
Claims31
A method of producing formate in a recombinant host, said method comprising:
- enzymatically converting 2-methyl-isocitrate to pyruvate in said recombinant host using a protein having methylisocitrate lyase activity;
- and
- enzymatically converting pyruvate to formate in said recombinant host using a protein having formate C-acetyltransferase activity.
5-9. (canceled)
A method of producing formate in a recombinant host, said method comprising:
- enzymatically converting lactate to pyruvate in said recombinant host using a protein having L-lactate dehydrogenase activity and a protein having lactate-malate transhydrogenase activity;
- and
- enzymatically converting pyruvate to formate in said recombinant host using a protein having formate C-acetyltransferase activity;
- or
- enzymatically converting L-homoserine to 2-oxobutyrate in said recombinant host using a protein having threonine ammonia-lyase activity and a protein having cystathionine γ-lyase activity;
- and
- enzymatically converting 2-oxobutyrate to formate in said recombinant host using a protein having formate C-acetyltransferase activity;
- or
- enzymatically converting CO2 to formate in said recombinant host using a protein having reductive NADP/NAPDH-dependent formate dehydrogenase activity.
(canceled)
15-22. (canceled)
A method of producing β-D-fructofuranose 6 phosphate in a recombinant host, said method comprising:
- enzymatically converting formyl-CoA and NADH to formaldehyde and NAD+ in said recombinant host using a protein having acetaldehyde dehydrogenase activity;
- enzymatically converting D-ribulose 5-phosphate and formaldehyde to hexulose 6-phosphate in said recombinant host using a protein having phosphoenolpyruvate carboxylase activity;
- and
- enzymatically converting hexulose 6-phosphate to β-D-fructofuranose 6 phosphate in said recombinant host using a protein having 6-phospho-3-hexuloisomerase activity.
(canceled)
28-29. (canceled)
(canceled)
33-39. (canceled)
A recombinant host comprising at least one exogenous nucleic acid encoding a methylisocitrate lyase and an anaplerotic enzyme.
44-45. (canceled)
A method for more efficiently recycling reduced electron carriers or fixing carbon in a recombinant host comprising:
- providing at least one microorganism capable of hydrogen oxidation, wherein the microorganism has an operable Calvin-Benson cycle;
- attenuating the Calvin Benson cycle in said microorganism;
- and
- utilizing the donated electrons or fixing carbon more efficiently than the microorganism having a Calvin Benson cycle.
49-51. (canceled)
Dependencies are inferred from the claim text.