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US 2018/0002687 A1Published application
Methods for Enhancing Bacterial Cell Display of Proteins and Peptides
AssigneeThe Regents of the University of California
InventorsPatrick Sean Daugherty, Jeffrey Rice
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Abstract
Methods of making and using bacterial display polypeptide libraries using circularly permuted OmpX (CPX) variants are disclosed. The invention further relates to methods for enhancing the display of proteins and peptides at the surface of bacteria by optimizing linkers and incorporating mutations at positions 165 and 166 of CPX.
Claims40
-42. (canceled)
A circularly permuted OmpX (CPX) variant comprising
- a linker joining the native N-terminus and native C-terminus of OmpX,
- wherein the CPX variant comprises a non-native N-terminus and a non-native C-terminus,
- wherein the linker is 4-8 residues in length and comprises a sequence X-Zn,
- wherein X is an amino acid selected from the group consisting of:
- serine, threonine, and proline,
- wherein each Z is independently any amino acid, and n is 2 to 4, and
- wherein at least one of the Z residues is independently selected from the group consisting of:
- lysine, arginine, glutamine, asparagine, or histidine.
5 more elements
Dependencies are inferred from the claim text.