Engineered multifunctional enzymes and methods of use
AssigneeDanisco US Inc.
InventorsZachary Q. Beck, Meredith K. Fujdala, Henrik Hansson, Thijs Kaper, Slavko Kralj, Amy D. Liu, Nils Egil Mikkelsen, Mats Sandgren
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Abstract
Provided are certain glycosyl hydrolase family 3 (GH3) beta-xylosidases engineered to acquire beta-glucosidase activities. Provided also are compositions comprising such multi-functional GH3 enzymes and methods of use or industrial applications thereof.
Claims20
An engineered beta-xylosidase of glycosyl hydrolyase family 3, comprising an amino acid sequence that is at least 80% identical to that of SEQ ID NO:2, further with at least one substitution at residue 87, 292, or 324, which residues are numbered in reference to the amino acid sequence of SEQ ID NO:3.
A polynucleotide encoding an engineered beta-xylosidase of glycosyl hydrolase family 3, having a polynucleotide sequence that is at least 70% identity to SEQ ID NO:1, and encodes an amino acid sequence having one or more substitution amino acid residues at amino acid residues 87, 292 or 324, which amino acid residues are numbered with reference to SEQ ID NO:3.
A method of producing an engineered GH3 beta-xylosidase polypeptide comprising an amino acid sequence that is at least 80% identical to SEQ ID NO:2 and with one or more substitution at amino acid residues 87, 292, or 324, which amino acid residues are numbered with reference to SEQ ID NO:3, comprising culturing the host cell of claim 15, under suitable conditions to produce the polypeptide.
Dependencies are inferred from the claim text.