Compositions and methods for protein glycosylation
AssigneeGlaxosmithkline Biologicals SA
InventorsJurgen HAAS, Julian IHSSEN, Michael Thomas KOWARIK, Torsten Franz SCHWEDE, Linda Christiane THÖNY-MEYER
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Abstract
Described herein are oligosaccharyl transferases for use in N-glycosylating proteins of interest in vitro and in host cells. Methods for using such oligosaccharyl transferases, nucleic acids encoding such oligosaccharyl transferases, and host cells comprising such oligosaccharyl transferases are also provided herein. Glycoconjugates generated by using such oligosaccharyl transferases are also provided herein.
Claims15
1-16. (canceled)
A recombinant N-oligosaccharyl transferase, wherein the recombinant N-oligosaccharyl transferase (N-OST) can detectably link an oligosaccharide or polysaccharide lacking an N-acetyl sugar at the reducing end to a carrier protein at an N-glycosylation consensus sequence wherein one or more amino acids selected from the group consisting of Y77, S80, 5196, N311, Y462, K482, D483 and G477 of Pg1BCj are modified .
Dependencies are inferred from the claim text.