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US 2018/0002678 A1Published application
Polypeptides Having Laccase Activity and Polynucleotides Encoding Same
AssigneeNovozymes, Inc.
InventorsYe Liu, Lan Tang, Junxin Duan, Yu Zhang
Life of this patent
Estimating the term…
Abstract
The present invention relates to isolated polypeptides having laccase activity and polynucleotides encoding the polypeptides and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Claims5
1-11. (canceled)
A process for degrading a cellulosic material, comprising:
- treating the cellulosic material with an enzyme composition in the presence of the polypeptide having laccase activity selected from the group consisting of:
- (a) a polypeptide having at least 60% sequence identity to the mature polypeptide of SEQ ID NO:
- 2, SEQ ID NO:
- 6, SEQ ID NO:
- 8, SEQ ID NO:
- 10, SEQ ID NO:
- 12, SEQ ID NO:18, or SEQ ID NO:
- 22;
- at least 65% sequence identity to the mature polypeptide of SEQ ID NO:
- 34 or SEQ ID NO:
- 36;
- at least 70% sequence identity to the mature polypeptide of SEQ ID NO:
- 14, SEQ ID NO:
- 16, SEQ ID NO:
- 20, SEQ ID NO:
- 26, SEQ ID NO:
- 30, or SEQ ID NO:
- 32;
- at least 75% sequence identity to the mature polypeptide of SEQ ID NO:
- 28;
- or at least 85% sequence identity to the mature polypeptide of SEQ ID NO:
- 4;
- (b) a polypeptide encoded by a polynucleotide that hybridizes under at least medium-high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
- 1, SEQ ID NO:
- 5, SEQ ID NO:
- 7, SEQ ID NO:
- 9, SEQ ID NO:
- 11, SEQ ID NO:17, SEQ ID NO:
- 21, SEQ ID NO:
- 33, or SEQ ID NO:
- 35, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii);
- or at least high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
- 13, SEQ ID NO:
- 15, SEQ ID NO:
- 19, SEQ ID NO:
- 25, SEQ ID NO:
- 27, SEQ ID NO:
- 29, or SEQ ID NO:
- 31, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii);
- or at least very high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
- 3, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii);
- (c) a polypeptide encoded by a polynucleotide having at least 60% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 1, SEQ ID NO:
- 5, SEQ ID NO:
- 7, SEQ ID NO:
- 9, SEQ ID NO:
- 11, SEQ ID NO:17, or SEQ ID NO:
- 21, or the cDNA sequences thereof;
- at least 65% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 33 or SEQ ID NO:
- 35, or the cDNA sequences thereof;
- at least 70% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 13, SEQ ID NO:
- 15, SEQ ID NO:
- 19, SEQ ID NO:
- 25, SEQ ID NO:
- 29, or SEQ ID NO:
- 31, or the cDNA sequences thereof;
- at least 75% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 27 or the cDNA sequence thereof;
- or at least 85% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 3 or the cDNA sequence thereof;
- (d) a variant of the mature polypeptide of SEQ ID NO:
- 2, SEQ ID NO:
- 4, SEQ ID NO:
- 6, SEQ ID NO:
- 8, SEQ ID NO:
- 10, SEQ ID NO:
- 12, SEQ ID NO:
- 14, SEQ ID NO:
- 16, SEQ ID NO:
- 18, SEQ ID NO:
- 20, SEQ ID NO:
- 22, SEQ ID NO:
- 26, SEQ ID NO:
- 28, SEQ ID NO:
- 30, SEQ ID NO:
- 32, SEQ ID NO:
- 34, or SEQ ID NO:
- 36 comprising a substitution, deletion, and/or insertion at one or more (e.g., several) positions;
- and
- (e) a fragment of the polypeptide of (a), (b), (c), or (d) that has laccase activity.
79 more elements
13-21. (canceled)
Dependencies are inferred from the claim text.