Method for assisting in evaluating pluripotent stem cell without stains, storage medium, and operation device

A non-transitory storage medium having stored therein a program that causes a computer to execute a process comprising:

• receiving signals of images of a biological sample, the images being captured by a microscope that converts a phase distribution into an image intensity distribution;
• calculating a first phase distribution of the biological sample from the image signals;
• extracting a region having a phase amount that is not less than a specified phase amount from the first phase distribution;
• and
• generating evaluation information by using the region having a phase amount that is not less than the specified phase amount, that is an indicator used when a user evaluates a state of the biological sample, and outputting the evaluation information, wherein
• the specified phase amount is a phase amount of a mitochondrion in a somatic cell.

An operation device comprising:

• a communication unit that receives signals of images of a biological sample and outputs data to a display medium, the images being captured by a microscope that converts a phase distribution into an image intensity distribution;
• and
• a calculator that calculates a first phase distribution of the biological sample from the image signals, wherein
• the calculator extracts a region having a phase amount that is not less than a specified phase amount from the first phase distribution, and generates evaluation information by using the region having a phase amount that is not less than the specified phase amount, that is an indicator used when a user evaluates a state of the biological sample;
• the communication unit outputs the evaluation information to the display medium;
• and
• the specified phase amount is a phase amount of a mitochondrion in a somatic cell.