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US 2018/0002669 A1Published application
Ex vivo proliferation of epithelial cells
AssigneePropagenix Inc.
InventorChengkang ZHANG
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Abstract
The technology relates in part to methods and compositions for ex vivo proliferation and expansion of epithelial cells.
Claims30
A method for proliferating epithelial cells ex vivo, comprising:
- expanding the number of cells in an originating epithelial cell population comprising differentiated epithelial cells under serum-free and feeder-cell free expansion culture conditions, thereby generating an expanded epithelial cell population, wherein the expansion culture conditions comprise one or more agents that inhibit transforming growth factor beta (TGF-beta) signaling in the population, and
- activating telomerase reverse transcriptase in the epithelial cell population.
A population of ex vivo expanded epithelial cells produced by a method comprising:
- expanding the number of cells in an originating epithelial cell population comprising differentiated epithelial cells under serum-free and feeder-cell free expansion culture conditions, thereby generating an expanded epithelial cell population, wherein the expansion culture conditions comprise one or more agents that inhibit transforming growth factor beta (TGF-beta) signaling in the population, and
- activating telomerase reverse transcriptase in the epithelial cell population.
A method for genetically modifying cells, comprising contacting cells with one or more agents that introduce a genetic modification to the cells, wherein the cells have been expanded by a method comprising:
- expanding the number of cells in an originating epithelial cell population comprising differentiated epithelial cells under serum-free and feeder-cell free expansion culture conditions, thereby generating an expanded epithelial cell population, wherein the expansion culture conditions comprise one or more agents that inhibit transforming growth factor beta (TGF-beta) signaling in the population, and
- activating telomerase reverse transcriptase in the epithelial cell population.
Dependencies are inferred from the claim text.