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US 2018/0002668 A1Published application
Efficient induction of definitive endoderm from pluripotent stem cells
AssigneesNovo Nordisk A/S, Takara Bio Europe AB
InventorsNina Funa, Katja Hess, Jenny Ekberg, Henrik Semb
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Abstract
The present invention relates to a method to differentiate pluripotent stem cells to a primitive streak cell population, in a stepwise manner for further maturation to definitive endoderm.
Claims17
A method for differentiation of stem cells into definitive endoderm comprising the steps of:
- incubating stem cells in a medium comprising at least 2 μM CHIR, wherein activin A is not present;
- and
- subsequently incubating stem cells in a medium comprising activin A.
A method for inducing primitive streak cells from embryonic stem cells by using CHIR in a concentration of at least 2 μM in the culture medium.
A method for inducing definitive endoderm cells from embryonic stem cells by using CHIR in a concentration in the range of 3.5-7 μM.
Dependencies are inferred from the claim text.