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US 2018/0002666 A1Published application
Cell separation apparatus
AssigneeTissue Genesis, Inc.
InventorsRolf WOLTERS, Anthony YANG, Josh NELSON, Stuart K. WILLIAMS
Life of this patent
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Abstract
Systems and methods herein are directed towards the separation of biologic material to obtain a target cell volume and/or cell concentration for harvesting. The target volume and/or concentration of cells may be obtained through a single cycle via three chambers, or by repeated cycles through one or more chambers to dilute the digestive enzymes used in the process and concentrate the harvestable cell volume to a predetermined target.
Claims20
A system for obtaining a target cell volume, comprising:
- a first chamber comprising a first separation mechanism and configured to separate a volume of biological material into a first retained volume and a first transfer volume;
- a second chamber in fluid communication with the first chamber and configured to:
- receive the first transfer volume from the first chamber and
- separate, in response to receiving the first transfer volume, a second transfer volume and a second retention volume from the first transfer volume;
- a third chamber comprising a first side and a second side, wherein the first side is in fluid communication with the second chamber and configured to:
- receive the second transfer volume;
- and
- separate a third transfer volume and a waste volume from the second transfer volume;
- a pump in fluid communication with at least the third chamber, wherein the pump is configured in a first state to establish a horizontal flow of the second transfer volume from the first side of the third chamber to the second side of the third chamber;
- a waste collection repository in fluid communication with the second side of the third chamber via a first coupling and configured to receive the waste volume;
- and
- a product collection repository in fluid communication with the second side of the third chamber via a second coupling and configured to receive the third transfer volume, wherein the third transfer volume comprises a predetermined volume of cells and fluid.
9 more elements
A method of obtaining a target cell volume, comprising:
- separating, via a first separation mechanism in a first chamber of a cell separation apparatus, and when a pump coupled to the cell separation apparatus is in an activated state, a starting volume of biological materials into a first transfer volume and a first retained volume;
- transferring the first transfer volume into a second chamber of the cell separation apparatus, wherein the second chamber is fluidly coupled to the second chamber;
- separating, via a second separation mechanism coupled to the second chamber, the first transfer volume into a second transfer volume and a second retained volume;
- transferring the second transfer volume into a third chamber of the cell separation apparatus, wherein the second chamber is fluidly coupled to the third chamber;
- separating, via a third separation mechanism coupled to the third chamber, the second transfer volume into a first cell volume, wherein separating the second transfer volume comprises performing at least one separation cycle;
- obtaining, subsequent to the at least one separation cycle, the first cell volume in the product collection repository.
3 more elements
A system for obtaining a target cell volume, comprising:
- a first chamber comprising a first separation mechanism, wherein the first separation mechanism is configured to separate a volume of biological material into a volume of fibrous tissue and lipids and a first transfer volume;
- a second chamber in fluid communication with the first chamber, wherein the second chamber comprises a second separation mechanism configured to receive the first transfer volume from the first chamber and separate out a second transfer volume;
- a third chamber in fluid communication with the second chamber;
- a waste repository in fluid communication with the third chamber;
- a product collection repository in fluid communication with the third chamber;
- and
- a pump, wherein the third chamber comprises a third separation mechanism configured to separate the second transfer volume into a cell volume comprising solids larger than a pore size of the third separation mechanism and a waste volume comprising solids smaller than the pore size of the third filter, and wherein the pump in a first activated state establishes a flow through the third chamber such that the cell volume is transferred to the product collection repository and the waste volume is transferred to the waste repository.
4 more elements
Dependencies are inferred from the claim text.