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US 2018/0002662 A1Published application
Purification of germ stem cells by targeting mrp9
AssigneePresident and Fellows of Harvard College
InventorsMichael B. Schultz, Michael S. Bonkowski, David A. Sinclair
Life of this patent
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Abstract
Provided herein are methods and compositions for the purification and detection of germ stem cells (e.g., oogonial stem cells) based on expression of MRP9.
Claims61
A method for purifying a germ stem cell (GSC), the method comprising:
- (a) contacting a sample comprising the GSC with an anti-MRP9 antibody that specifically binds to MRP9 and that does not specifically bind to DDX4;
- (b) incubating the sample under conditions such that the anti-MRP9 antibody forms a complex with an MRP9 protein expressed on the surface of the GSC;
- and
- (c) separating the GSC from other material present in the sample.
1 more element
A method for generating a culture of germ stem cells (GSCs), the method comprising:
- (a) contacting a sample comprising the GSCs with anti-MRP9 antibodies that specifically bind to MRP9 and that do not specifically bind to DDX4;
- (b) incubating the sample under conditions such that the anti-MRP9 antibodies form complexes with MRP9 proteins expressed on the surface of the GSCs;
- (c) separating the GSCs from other material present in the sample;
- and
- (d) culturing the GSCs.
2 more elements
A method for detecting a germ stem cell (GSC), the method comprising:
- (a) contacting a sample comprising the GSC with an anti-MRP9 antibody that specifically binds to MRP9 and that does not specifically bind to DDX4;
- (b) incubating the sample under conditions such that the anti-MRP9 antibody forms a complex with an MRP9 protein expressed on the surface of the GSC;
- and
- (c) detecting the GSC by detecting the complex.
1 more element
Dependencies are inferred from the claim text.