Cell culturing method and kit
AssigneeUbe Industries, Ltd.
InventorsMasahiko HAGIHARA, Motohisa SHIMIZU, Yukinori WADA
Life of this patent
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Abstract
The present invention relates to a cell culturing method and kit. More specifically, it relates to a cell culturing method and kit using a support that is exposed to the air. It further relates to a method of culturing cells by allowing them to migrate onto a porous polyimide film.
Claims37
A cell culturing method including:
- (1) a step of supporting cells on one or more sheet-like porous supports,
- (2) a step of applying a medium to the sheet-like porous support supporting the cells and wetting the sheet-like porous support with the medium, with the medium included in some or all of the pores of the sheet-like porous support,
- (3) a step of disposing the sheet-like porous support in a culturing vessel that houses medium, in such a manner that all or a portion of the surface of the sheet-like porous support wetted with the medium is exposed to a gas phase, and
- (4) a step of setting the culturing vessel in an incubator and culturing the cells,
- wherein the wetted state of the sheet-like porous support surface and interior is maintained throughout the culturing.
A method of culturing cells by allowing them to migrate onto a porous polyimide film, the method including contacting a porous polyimide film with a cell culture substrate in which cells are being cultured to allow the cells to migrate from the cell culture substrate onto the porous polyimide film, and culturing the cells that have been allowed to migrate onto the porous polyimide film.
A method of culturing cells by allowing them to migrate onto a porous polyimide film, the method including contacting the top surface or bottom surface, or both, of a first porous polyimide film on which cells are being cultured, with a second porous polyimide film on which cells are not being cultured, to allow the cells to migrate from the first porous polyimide film to the second porous film, and culturing the cells that have been allowed to migrate to the second porous polyimide film.
A method of culturing cells by allowing them to migrate onto a porous polyimide film, the method including contacting a cell-containing biological sample with the top surface or bottom surface, or both, of a porous polyimide film, to allow the cells to migrate from the biological sample to the porous polyimide film, and culturing the cells that have been allowed to migrate to the porous polyimide film.
Dependencies are inferred from the claim text.