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US 2018/0002652 A1Published application
Apparatuses and methods for electroporation
AssigneeZymergen Inc.
InventorsJohn Cesarek, Travis Lee, Richard Hansen
Life of this patent
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Abstract
Disclosed are apparatuses, systems, and methods for performing electroporation.
Claims27
An apparatus comprising:
- a plurality of cuvette holders, wherein each cuvette holder is configured to hold a plurality of cuvettes and includes a bottom having a thermal conductivity greater than 150 W/(m K), a sidewall having a thermal conductivity greater than 150 W/(m K), and a plurality of cuvette positioning features, wherein:
- the sidewall and the bottom are in thermal connection with each other and at least partially define an internal volume of the cuvette holder, and
- each cuvette positioning feature is configured to restrain a movement of a cuvette that is inserted into the internal volume such that a portion of each cuvette that is inserted into the internal volume is in thermal connection with the sidewall;
- a plate having a thermal conductivity greater than 150 W/(m K) and including a first surface and a plurality alignment features, wherein:
- each alignment feature is configured to restrain a movement of a cuvette holder that is positioned on the first surface with respect to the first surface, and
- the bottom of each cuvette holder that is placed on the first surface and restrained by at least one alignment feature is in thermal connection with the first surface;
- and
- a fluid flowpath in thermal connection with the first surface.
5 more elements
A method of electroporation, the method comprising:
- cooling a plurality of cuvettes containing a solution and inserted into a cuvette holder that is positioned on a first surface of a plate by flowing a heat transfer fluid through a fluid flow path that is in thermal connection to the first surface, wherein a bottom of the cuvette holder is in thermal connection with the first surface and with a sidewall of the cuvette holder, and wherein the sidewall is in thermal connection with a portion of each cuvette, and
- driving, after cooling the plurality of cuvettes to a first temperature, a current alternately to each cuvette, wherein the current flows through a second portion of a cuvette, through the solution of that cuvette, and through a portion of the cuvette to a ground.
Dependencies are inferred from the claim text.