Antibodies to matrix metalloproteinase 9
AssigneeGilead Biologics, Inc.
InventorsJoanne I. ADAMKEWICZ, Victoria SMITH, Zung THAI, Michael J. HAWKINS
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Abstract
The present disclosure provides compositions and methods of use involving binding proteins, e.g., antibodies and antigen-binding fragments thereof, that bind to the matrix metalloproteinase-9 (MMP9) protein (MMP9 is also known as gelatinase-B), such as where the binding proteins comprise an immunoglobulin (Ig) heavy chain (or functional fragment thereof) and an Ig light chain (or functional fragment thereof).
Claims20
A method of inhibiting Matrix Metalloproteinase 9 (MMP9) activity in a subject in need thereof, comprising:
- administering to the subject an effective amount of a pharmaceutical composition comprising an isolated antibody or fragment thereof that binds to MMP9, and a pharmaceutically acceptable excipient.
A method of detecting or monitoring MMP9 activity, comprising:
- contacting a sample with MMP9 binding protein,
- assessing the presence or absence of MMP9 binding protein-MMP9 complex;
- wherein the MMP9 binding protein comprises a VH region comprising a CDR with an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 13, 14, 15, 34, 35, 36 and 47;
- and a light chain variable (VL) region having a complementary determining region (CDR) with an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 16, 17, 18, 37, 38, 39, 42, 43, 44, and 48;
- whereby the absence of the MMP9 binding protein-MMP9 complex indicates the sample does not have the MMP9 activity, whereby the presence of the MMP9 binding protein-MMP9 complex indicates the sample has the MMP9 activity.
A MMP9 binding protein comprises a heavy chain variable (VH) region comprising a complementary determining region (CDR) with an amino acid sequence of SEQ ID NOs:
- 34, 35, 36 and 47;
- and a light chain variable (VL) region having a complementary determining region (CDR) with an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 37, 38, 39, 42, 43, 44, and 48.
Dependencies are inferred from the claim text.